in the present study, pathogenicity of four native strains of entomopathogenic fungus; metarhizium anisopliae, was studied against adult stage of ixodes ricinus. for this purpose a total number of 180 adult ticks were examined in triplicate. thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. samples were incubated in separate pet...

BACKGROUND Metarhizium anisopliae strain IRAN 437C is one of the most virulent fungal isolates against house fly, Musca domestica. The objective of this study was to determine the interaction of this isolate with sublethal doses of spinosad against housefly. METHODS In adult bioassay, conidia of entomopathogenic fungus were applied as inoculated bait at 10(5) and 10(7) spore per gram and spin...

The exopolysaccharide (EPS) separated from the entomopathogenic fungus Metarhizium anisopliae was determined by gel permeation chromatography to be homogeneous. The high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAE-PAD) showed a content of monosaccharides D-galactosamine and D-fucose at a molar ratio of about 2:1. The results obtained from Fourier transform...

In this study, Metarhizium rileyi isolates (M. NIPHM, M. MTCC 4254 and 10395) were evaluated for their compatibility with other entomopathogenic fungi viz. anisopliae NBAIR (Ma-35), commercial formulation of anisopliae, (Local), Beauveria bassiana ITCC 7126, B. (Local) Verticillium lecanii neem based formulations- (azadirachtin 0.03%EC) 0.15%EC) along synthetic insecticide spinetoram 11.7%SC. N...

Entomopathogenic fungi are naturally occurring pathogens of some insects that produce enzymes capable of penetrating into an insect’s cuticle. Chitinases are thought to be responsible for entomopathogenic fungi’s ability to degrade cuticle. Primers were designed for the chi3 gene which encodes a chitinase CHIT30, from the mRNA transcript present in Metarhizium anisopliae. These primers were una...

We present two cases of keratitis due to Metarhizium anisopliae in geographically separated areas of the United States. The isolates were microscopically similar but morphologically different and were identified by ribosomal DNA sequencing. Both isolates had low minimum inhibitory concentration (MIC) values to caspofungin and micafungin, but high MIC values to amphotericin B. The morphologic an...