Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity Songklanakarin J. Sci. Technol., 2004, 26(2) : 211-219 The use of the stable free radical diphenylpicrylhydrazyl (DPPH) to estimate the activity of antioxidants is reviewed. Current applications of the method are examined, particularly the use of the parameter EC50 (substrate concen...

As plants produce a huge amount of antioxidants they can represent a source of new compounds with antioxidant activities. From this point of view, the main goal of this research was to study the antioxidant activities of the Ficus carica through the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging method. Since DPPH assay has been largely used as a quick, reliable, and reproducible parame...

1,1-dipheny-2-picrylhydrazyl (DPPH) scavenging activities of the 80% methanolic leaf extracts of three cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Mai-Men-Dong (Liriope spicata L.) are analyzed by spectrophotometry. The concentrations required for 50% inhibition (IC 50 ) of DPPH radicals were 81.08, 96.97, and 53.78 μg/mL, respectively. The methanolic extracts were further partit...

Optimization of phenolic extraction from Orthosiphon stamineus was carried out in present study by investigating the effects of ethanol concentration (0-100%, v/v), extraction time (60-300 min) and extraction temperature (25-65oC) on the phenolic recovery using single factor experiment. Total phenolic content (TPC), total flavonoid content (TFC) and condensed tannins content (CTC) were used for...

We deyeloped a simple screening method for antioxidant-producing strains using 1,1-diphenyl-2picrylhydrazyl (DPPH) and screened bacteria from 16 kinds of marine animals that lived in different enyironments in the sea to find 112 bacterial iso]ates producing antioxidants. Guided by decoloration of DPPH sprayed on silica gel TLC, uric acid, indole, 3,4-dimethoxyphenol, and 3-hydroxyindolin-2-one ...

Electron-transfer disproportionation of a 2,2-diphenyl-1-picrylhydrazyl radical (DPPH˙) occurred in the presence of Sc(3+) acting as a strong Lewis acid in deaerated acetonitrile. In contrast, in the case of weaker Lewis acids than Sc(3+), such as Mg(2+) and Li(+), external protons from acetic acid were required for the disproportionation of DPPH˙ to occur.

Strawberries can augment plasma antioxidant activity, but this may be confounded by selection of methods, time of blood sampling and concomitant dietary restrictions. We examined the effect of strawberry consumption on ferric reducing ability (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (DPPH-test) of native and non-urate plasma in healthy subjects on their usual ...

In this article, a DPPH·-luminol chemiluminescence (CL) system was reported and the CL mechanism was discussed according to the CL kinetic properties after sequence injecting DPPH· into the DPPH·-luminol reaction mixture. It was observed that scutellarin could inhibit the CL response of the DPPH·-luminol system. Based on this observation, a simple and rapid flow injection CL method was develope...