A 1.2-kilobase pair fragment of the 5' upstream region of a potato wound-inducible gene (wun1) was fused to different marker genes (wun1-CAT, wun1-NPTII). Stable integration of a wun1-CAT chimeric gene into the tobacco genome led to a high wound-inducible chloramphenicol acetyltransferase activity in leaves. Transient expression experiments in potato protoplasts showed that wun1 carries a stron...

Plasmid vectors containing both a selectable marker for plant transformation (kanamycin resistance) and a second, directly adjacent, divergent promoter for the transcription of inserted DNA fragments have been constructed. These vectors make use of a small (479 bp) dual-promoter DNA fragment, originally isolated from the T-DNA of Agrobacterium tumefaciens, fused to the neomycin phosphotransfera...

Abstract Given the availability of technological solutions and guidelines for safe drinking water, direct potable reuse reclaimed water has become a promising option to overcome severe lack in arid regions. However, growing awareness presence antibiotic-resistant bacteria (ARB) antibiotic resistance genes (ARG) corresponding raw wastes led new safety concerns. This study investigated fate ARB i...

In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

BACKGROUND The future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown GM lines. In this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes β-glucuronidase (uidA) and neomycin phosphotransferase ...

Abstract Background One of the parameters required for assessment food and feed safety is detection identification genetically modified organisms. Legislation in some countries necessitates quantification modification samples. Scientists have raised concern about antibiotic resistance marker (ARM) genes used transformation crops intended human animal consumption. In present work two molecular a...

Whitefly (Bemisia tabaci) is a polyphagous insect that causes huge damage in several horticultural crops, including tomato, by sucking nutrients from the phloem and transmitting viruses. Whiteflies are particularly difficult to manage use of chemicals remains common practice, which development insecticidal resistance. Thus, there considerable interest introduction resistance whitefly classical ...