Three different classes of Fc receptors for IgG (Fc gamma R) are currently distinguished in humans, of which polymorphonuclear phagocytes (PMN) normally express both low-affinity receptor classes--Fc gamma RII (CD32) and Fc gamma RIII (CD16). During therapy with granulocyte colony-stimulating factor (G-CSF), neutrophils from patients with various malignancies and different hematologic disorders...

In the present work, we developed the Fc-III tagged protein expression system for protein purification and detection. The Fc-III sequence encodes for a 13 residue peptide and this peptide is cyclized by disulfide bond formation when the fusion protein is expressed. The Fc-III-fusion proteins selectively bind to immunoglobulin Fc domains (IgG-Fc) expressed from E. coli. We showed the efficient p...

In mouse mast cells, both Fc e RI and Fc g RIII are abg 2 tetrameric complexes in which different a chains confer IgE or IgG ligand recognition while the signaling FcR b and g chains are identical. We used primarily noninvasive techniques (changes in body temperature, dye extravasation) to assess systemic anaphylactic responses in nonanesthetized wild-type, Fc e RI a chain 2 / 2 and FcR g chain...

We demonstrate, using a recombinant truncated Fc gamma RII molecule as a probe, the presence of anti-Fc gamma R antibodies in several strains of autoimmune mice. Affinity chromatography on a truncated Fc gamma R column of pooled sera from aged NZB females resulted in isolation of 16 micrograms of IgM per ml of serum, approximately 2% of the total IgM; no anti-Fc gamma R IgM was found in sera fr...

Monoclonal antibodies (MoAbs) have been prepared recently that recognize the three cell-surface receptors for the Fc portion of immunoglobulin (Ig), termed Fc gamma RI (MoAb 32.2), Fc gamma R II (MoAb IV-3), and Fc gamma R III (MoAb 3G8) that are expressed on selected subsets of non-T lymphocyte peripheral blood leukocytes. In the blood, Fc gamma R I is expressed exclusively on monocytes and ma...

A bunch of factors including the limited fossil resources and rising of fossil fuel price have been causes moving to create the new structure that is based on providing energy security, and protect the environment. One of the alternatives is the fuel cell (FC). Maximum power point tracker has an important role to increase the efficiency of the FC systems. One of the difficulties in maximum powe...

The possibility that fusicoccin (FC) binds to plasma membrane-associated ATPases of oat (cv. Victory) roots has been examined. Specific FC-binding in vitro is localized primarily on plasma membrane-enriched fractions. This FC-binding is greatly reduced by pretreatment of the membrane vesicles at temperatures above 45 C or with trypsin, and the same treatments cause the release of already bound ...

Human Fcy receptors (FcyRs) for the Fc portion of IgG are major mediators of the adaptive immune response. Crystal structures of their extracellular domains were recently solved and shown to obey a common fold, resulting in a heart-shaped structure. Together with the multifunctional Fc fragment, whose structure was deciphered in the 1970s, the complex of an FcyR with Fc was recently crystallize...

IgG1 Fc is a remarkable molecule, capable of initiating pro-inflammatory or antiinflammatory responses by binding to Fc receptors. IgG1 Fc has a conserved glycosylation site at N297. The presence of the carbohydrate at N297 is vital for proper Fc function. Intramolecular glycan-polypeptide interactions are thought to impose conformational restrictions on Fc that maintain binding-favorable inter...