Many studies using mammalian cellular and subcellular systems have demonstrated that polycyclic aromatic hydrocarbons, including benzo[a]pyrene (BaP), are metabolically activated by cytochrome P450s (CYPs). In order to evaluate the role of hepatic versus extra-hepatic metabolism of BaP and its pharmacokinetics, we used the hepatic cytochrome P450 reductase null (HRN) mouse model, in which cytoc...

Chemical-DNA adducts provide an integrated measure of exposure, absorption, bioactivation, detoxification, and DNA repair following exposure to a genotoxic agent. Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon (PAH), can be bioactivated by cytochrome P-450s (CYPs) and epoxide hydrolase to genotoxic metabolites which form covalent adducts with DNA. In this study, we utilize...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...

In vitro callogenesis was achieved from leaf explants of Boerhaavia diffusa Linn. on basal medium supplemented with different concentrations of BAP, NAA, Kn and IAA. Regeneration of shoot buds were observed on MS medium supplemented with BAP and NAA at different concentrations. In some of the replicates the shoot buds were elongated and terminated in flower buds. In vitro flowering occurred wit...

OBJECTIVES The aim of our study was to assess individual polycyclic aromatic hydrocarbon (PAH) exposure of workers coming from three different industrial branches by several parameters of external and internal exposure. By analysing the relationships between those markers the suitability of individual parameters [e.g. monohydroxylated phenanthrene (Phe) metabolites] for exposure surveillance sh...

BAP is a publicly available infrastructure for performing program verification and analysis tasks on binary (i.e., executable) code. In this paper, we describe BAP as well as lessons learned from previous incarnations of binary analysis platforms. BAP explicitly represents all side effects of instructions in an intermediate language (IL), making syntaxdirected analysis possible. We have used BA...

two experiments were performed for in vitro establishment, proliferation and shoot growth in axillary bud explants of sebri pear cultivar. in the first experiment, the effect of different concentrations of iba(indole-3-butyric acid) on explants’ establishment was evaluated. in a second experiment, the influence of several combinations of pgrs (plant growth regulators)on shoot proliferation and ...