We employed quasielastic and static light scattering to measure apparent values of the mean hydrodynamic radii (Rh)app, molecular weights (Mapp), and radii of gyration (Rg)app in solutions containing mixed micelles composed of bile salts (cholate and taurochenodeoxycholate, both cholanoyl derivatives) and the glycoacyl chain detergent, octyl glucoside, with egg yolk phosphatidylcholine (EYPC) a...

The inner histone complex, extracted from chicken erythrocyte chromatin in 2 M NaCL AT pH 7.4, has been characterized by sedimentation equilibrium and sedimentation velocity. High speed sedimentation equilibrium studies indicate that in 2 M NaCl the inner histones are a weakly associating system with contributions from species ranging in molecular weight from dimer to octamer. The appearance of...

Zeaxanthin is a xanthophyll pigment that is regarded as one of the best carotenoids for the prevention and treatment of degenerative diseases. In the worldwide natural products market, consumers prefer pigments that have been produced from biological sources. In this study, a Dunaliella tertiolecta strain that has 10-15% higher cellular zeaxanthin content than the parent strain (zea1), was obta...

Density and Ultrasonic velocity Sucrose in 1M NaCl at 298.15K, 303.15K, 308.15K and 313.15K have been measured. Various ultrasonic and solution parameters such as adiabatic compressibility, change and relative change in adiabatic compressibility, acoustic impedance, available volume and free length have been calculated using these data. The present investigation has been used to exploit the pos...

1. The equations which serve to predict the injury of tissue in 0.52 M NaCl and in 0.278 M CaCl(2) and its subsequent recovery (when it is replaced in sea water) also enable us to predict the behavior of tissue in mixtures of these solutions, as well as its recovery in sea water after exposure to mixtures. 2. The reactions which are assumed in order to account for the behavior of the tissue pro...

Transient receptor potential (TRP) subfamily M member 5 (TRPM5) cation channel is involved in sensing sweet, bitter, umami, and fat taste stimuli, complex-tasting divalent salts, and temperature-induced changes in sweet taste. To investigate if the amiloride- and benzamil (Bz)-insensitive NaCl chorda tympani (CT) taste nerve response is also regulated in part by TRPM5, CT responses to 100 mM Na...

BsiHKA I is a type II restriction endonuclease from a Bacillus stearothermophilus strain isolated from soil according to (1). fisiHKA I in 11 culture (4.7 g wet cells) was purified by a DEAEsephacel column (30 ml bed volume). Enzyme eluted at about 0.3 M NaCl was dialysed against buffer A (1) and loaded onto a heparin column (8 ml bed volume). Enzyme eluted at 0.4—0.5 M NaCl was dialysed agains...

The nucleus of the solitary tract (NTS) is the primary site of visceral afferents to the central nervous system. In the present study, we investigated the effects of lesions in the commissural portion of the NTS (commNTS) on the activity of vasopressinergic neurons in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, plasma vasopressin, arterial pressure, water intake, and sod...

Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitro RNA synthesis in an amount proportional to the fraction of exposed D...