Extensive efforts have been made to develop anti-HIV drugs from potential HIV-1 protease inhibitor1'2) since the protease inhibitors have been proven to produce immature and non-infectious viruses3'4). In order to find HIV-1 protease inhibitors and study the kinetics of inhibition of the protease, many assay methods have been devised using the protease produced by genetic engineering techniques...

Although many human immunodeficiency virus type 1 (HIV-1)-infected persons are treated with multiple protease inhibitors in combination or in succession, mutation patterns of protease isolates from these persons have not been characterized. We collected and analyzed 2,244 subtype B HIV-1 isolates from 1,919 persons with different protease inhibitor experiences: 1,004 isolates from untreated per...

protease 2a (2apro) of coxsackievirus b3 (cvb3) plays a major role in viral replication. in case of infection, viral proteins are being synthesized from viral mrna using host biosynthesis machinery. 2apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4g. the enzyme plays an essential role in viral replica...

Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...

screening and identification of moderately halophilic protease producing bacteria from different regions of maharloo, a hypersaline lake in the southern area of iran, were the objectives of this study. in the preliminary screening, 16 isolates exhibited proteolytic activity on saline skim milk agar. all isolates were able to grow comfortably in the media containing 7-15% of salt. protease activ...

A model for the classification of 70 HIV-1 protease crystal structure binding pockets to one of its complexed FDA approved protease inhibitors utilizing Random Forest has been developed. 456 chemical descriptors of the binding pocket of each crystal structure have been computed and are used to develop the classification model. Simulations were performed to determine the optimal Random Forest mo...