A series of α-ketooxazoles incorporating electrophiles at the C5 position of the pyridyl ring of 2 (OL-135) and related compounds were prepared and examined as inhibitors of fatty acid amide hydrolase (FAAH) that additionally target the cytosolic port Cys269. From this series, a subset of the candidate inhibitors exhibited time-dependent FAAH inhibition and noncompetitive irreversible inactivat...

Polyacrylamide gel genome electropherotyping and negative-stain electron microscopic studies, along with immunofluorescent staining and immune electron microscopy reactions, indicate that rabbit syncytium virus has the morphologic, genomic, and antigenic attributes of a Kemerovo serogroup orbivirus.

A systematic study of the structure-activity relationships of 2b (OL-135), a potent inhibitor of fatty acid amide hydrolase (FAAH), is detailed targeting the C2 acyl side chain. A series of aryl replacements or substituents for the terminal phenyl group provided effective inhibitors (e.g., 5c, aryl = 1-napthyl, Ki = 2.6 nM), with 5hh (aryl = 3-ClPh, Ki = 900 pM) being 5-fold more potent than 2b...

An analysis was made of the neutralizing antibody repertoire, for influenza virus hemagglutinin (HA) of transgenic mice expressing a human immunoglobulin mu (IgH) minigene, by monoclonal antibody (MAb) selection and sequencing of the HA genes of X31 (H3N2 subtype) laboratory variants. Whereas previously reported laboratory variants, selected in ovo with high-affinity murine MAbs of the IgG clas...

To cleave blood group A immunodeterminants from erythrocytes (Hoskins, L. C., Larson, G., and Naff, G. B. (1995) Transfusion 35, 813-821), we purified and characterized alpha-N-acetylgalactosaminidase (EC 3.2.1.49) activity from culture supernatants of the human fecal bacterium Ruminococcus torques strain IX-70. Three isoforms separated during hydrophobic interaction chromatography. Hydroxyapat...

H.-W. Hübers,1,2,* S. G. Pavlov,1 S. A. Lynch,3 Th. Greenland,4 K. L. Litvinenko,5 B. Murdin,5 B. Redlich,6 A. F. G. van der Meer,6 H. Riemann,7 N. V. Abrosimov,7 P. Becker,8 H.-J. Pohl,9 R. Kh. Zhukavin,10 and V. N. Shastin10 1Institute of Planetary Research, German Aerospace Center (DLR), Rutherfordstrasse 2, 12489 Berlin, Germany 2Institut für Optik und Atomare Physik, Technische Universität...

One of the major causes of carcinogenesis is loss of genome stability. RAD51 in process of homologous recombination (HR) played crucial role in maintenance integrity of genome through initiate of DNA double strand breaks repair. Presence of single nucleotide polymorphism (SNP) in RAD51 gene could change the capacity of DNA repair and altered the response to damaging agents. Research on potentia...

a biofilm sequencing batch reactor with a volume of 1.42 m3, nylon nets providing a 4,140 m2/m3 support area for biofilms and an automated operation with 8 hour cycles was studied. the duration of the experiment was 135 days. removal efficiencies e≥ 80% were obtained for carbonaceous matter, producing an effluent with 31±26.8 mg/l of filtered cod, 7±3.6 mg/l of bod5 and 12±3.2 mg/l of toc. the ...