concentrations of zn, cu, cr, cd, pb and fe in surface marine sediments of the southern coast of sfax were studied in order to understand current metal contamination at the outlet of a mixed industrial and domestic wastewater effluent discharged into the seawater since 1953. we found that heavy metal concentrations (mg/kg dry weight) in surface marine sediments varied from 42 to 391 for zn, 15 ...

concentrations of zn, cu, cr, cd, pb and fe in surface marine sediments of the southern coast of sfax were studied in order to understand current metal contamination at the outlet of a mixed industrial and domestic wastewater effluent discharged into the seawater since 1953. we found that heavy metal concentrations (mg/kg dry weight) in surface marine sediments varied from 42 to 391 for zn, 15 ...

Nucleotide sequences of small-subunit rRNA (16S rRNA) are most commonly used for the identification and characterization of bacteria and their complex communities. However, 16S rRNA evolves slowly and is often not very convenient to resolve bacterial strains at the species level. We have therefore attempted to develop a rapid and more convenient system for bacterial identification using the gyr...

Horizontal gene transfer (HGT) is a ubiquitous genetic event in bacterial evolution, but it seldom occurs for genes involved in highly complex supramolecules (or biosystems), which consist of many gene products. The ribosome is one such supramolecule, but several bacteria harbor dissimilar and/or chimeric 16S rRNAs in their genomes, suggesting the occurrence of HGT of this gene. However, we kno...

BACKGROUND Anaplasma phagocytophilum is the causative agent of tick-borne fever in ruminants and human granulocytotropic anaplasmosis (HGA). The bacterium is able to survive for several months in immune-competent sheep by modifying important cellular and humoral defence mechanisms. Little is known about how different strains of A. phagocytophilum propagate in their natural hosts during persiste...

Bacteria are indispensable for the study of fundamental molecular biology processes due to their relatively simple gene and genome architecture. The ability to engineer bacterial chromosomes is quintessential for understanding gene functions. Here we demonstrate the engineering of the small-ribosomal subunit (16S) RNA of Mycoplasma mycoides, by combining the CRISPR/Cas9 system and the yeast rec...

the rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (pgpr). in this study, the isolation andidentification of rhizobacteria from orange (citrus sinensis) orchards using 16s rrna gene, as well asbiological and biochemical assays is reported. analysis of 16s rrna gene was confirmed bybiological and ...

The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer sets, OXY107F/OXY1313R commonly used to amplify oxygenic organisms, and PLA491F/OXY1313R, biased towards plastids of marine algae. Surprisingly, t...

It is evident from complete genome sequencing results that lateral gene transfer and recombination are essential components in the evolutionary process of bacterial genomes. Since this has important implications for bacterial systematics, the primary objective of this study was to compare estimated evolutionary relationships among a representative set of alpha-Proteobacteria by sequencing analy...