The present study was designed to characterize methicillin-resistant staphylococci from raw meat. A total of 126 meat samples were obtained from open markets between February and April, 2015. Antimicrobial susceptibility testing was carried out using the disc diffusion method. Molecular profiling was conducted using 16S rRNA, mecA, nuc, and PVL gene signatures were detected by polymerase chain ...

The Saccharomyces cerevisiae genes, RRP1 and SRD1, are involved in processing rRNA precursor species to mature rRNAs. We reported previously that the rrp1-1 mutation caused temperature-sensitive lethality, hypersensitivity to aminoglycoside antibiotics, and defective processing of 27S pre-rRNA to 25S and 5.8S mature rRNAs. A second-site suppressor of the rrp1-1 mutation, srd1, corrects all thre...

sarcocyst is one of the most important protozoa belonged to apicomplexa. this parasite is prevalent among warm blooded animals throughout the world. in the present work, sarcocystis gigantea was identified by amplification of 18s rrna gene using pcr- rflp. in this regard macroscopic cysts of sarcocystis were collected from esophagus and intra costal muscles of sheep in shahriar slaughterhouse. ...

BACKGROUND Broad-range 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection and identification of bacterial pathogens in clinical specimens from patients with a high suspicion for infection. However, prospective studies addressing the impact and clinical value of broad-range bacterial 16S rRNA gene amplification for diagnosis of acute infectious diseases in nonsel...

API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. Yersinia ferderiksenii and Yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. The 16S rRNA gene is considered...

INTRODUCTION Many studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. Molecular methods linked to PCR are more reliable and faster for identification of non- tuberculous mycobacteria (NTM). In this study molecular methods were used for identification and typing of NTM. MATERIALS AND METHODS Five hundred ml of 85 water samples was p...

This is the first report on the world that Pseudomonas psychrophila is a new etiological agent of disease in fish. A disease case occurred during the fall of 2017 in a rainbow trout farm in the north of Iran (Guilan province). Anorexia, lethargy and mortalities of 10% fish per week were observed in rainbow trout Oncorhynchus mykiss weighting 45-107g in three fresh water ponds of a farm on the n...

Correspondence between two distinct genetic traits, 16S rRNA gene sequences and repetitive element-sequence-based BOX-PCR DNA fingerprints, and antibiotic inhibition and resistance phenotypes was explored for a spatially explicit sample of Streptomyces from a prairie soil. There was no correspondence between 16S rRNA gene sequence groups and antibiotic phenotypes. However, 16S rRNA gene sequenc...

the lactic acid bacteria (lab) are an industrially important group of probiotic organisms that play an important role in human health by inhibiting harmful and pathogen bacteria growth, boosting immune function, and increasing resistance to infection.  this study aimed to isolate, identify, and biologically characterize probiotic lab strains from iranian traditional dairy products. a total of 2...