The ability of the liver, small intestine, and kidney to synthesize and subsequently eliminate dinitrophenyl-S-glutathione (DNP-SG), a substrate for multidrug resistance-associated protein 2 (Mrp2), was assessed in rats treated with glucagon-like peptide 2 (GLP-2, 12 μg/100 g b.wt. s.c. every 12 h for 5 consecutive days). An in vivo perfused jejunum model with simultaneous bile and urine collec...

The effects of mitochondrial uncoupling on skeletal muscle mitochondrial adaptation and maximal exercise capacity are unknown. In this study, rats were divided into a control group (CTL, n = 8) and a group treated with 2,4-dinitrophenol, a mitochondrial uncoupler, for 28 days (DNP, 30 mg·kg(-1)·day(-1) in drinking water, n = 8). The DNP group had a significantly lower body mass (P < 0.05) and a...

Objective The dorsal horn P2Y13 receptor is involved in the development of pain behavior induced by peripheral nerve injury. It is unclear whether the expression of proinflammatory cytokines interleukin (IL)-1β and IL-6 at the spinal dorsal horn are influenced after the activation of P2Y13 receptor in rats with diabetic neuropathic pain (DNP). Methods A rat model of type 1 DNP was induced by ...

There has been a great deal of curiosity about the new Doctor of Nursing Practice (DNP). I have been asked by my colleagues to share my story. "What is a DNP? What prompted you to seek a DNP? Why not a PhD? How will this degree help you in your career? Where will you practice?" Approached with so many questions and general curiosity, I decided to share my story.

We report the effects of ligand presentation on the binding of aqueous proteins to solid supported lipid bilayers. Specifically, we show that the equilibrium dissociation constant can be strongly affected by ligand lipophilicity and linker length/structure. The apparent equilibrium dissociation constants (K(D)) were compared for two model systems, biotin/anti-biotin and 2,4-dinitrophenyl (DNP)/...

Abstract. NMR-based analysis of metabolite mixtures provides crucial information on biological systems but mostly relies 1D 1H experiments for maximizing sensitivity. However, strong peak overlap spectra often is a limitation the inherently complex mixtures. Dissolution dynamic nuclear polarization (d-DNP) improves NMR sensitivity by several orders magnitude, which enables 13C metabolites at na...

Abstract. Dynamic nuclear polarization (DNP)-enhanced magic angle spinning (MAS) magnetic resonance (NMR) of biological systems is a rapidly growing field. Large signal enhancements make the technique particularly attractive for signal-limited cases, such as studies complex assemblies or at natural isotopic abundance. However, spectral resolution considerably reduced compared to ambient-tempera...

A series of non-aqueous solvents combined with the exogenous biradical bTbK are developed for DNP NMR that yield enhancements comparable to the best available water based systems. 1,1,2,2-tetrachloroethane appears to be one of the most promising organic solvents for DNP solid-state NMR. Here this results in a reduction in experimental times by a factor of 1000. These new solvents are demonstrat...

The concept of shared governance was used to frame the dialogue and decision-making process around the Doctor of Nursing Practice (DNP) at a leading School of Nursing. This article reports the process used to support dialogue around the DNP so that an informed decision could be made. The shared governance process promoted reflective conversation within the entire faculty and, in the end, suppor...

Background: Phenol and nitrophenol are common compounds found in different types of industrial wastewater known as serious threats to human health and natural environment. In this study, Daphnia magna was used to evaluate the effectiveness of &quot;baffle fixes film up flow sludge blanket filtration&quot; (BFUSBF) system in elimination of phenolic compounds from water. Methods: D. magna cult...