نتایج جستجو برای: 5 روش هیستوشیمیایی nadph دیافورزیز

تعداد نتایج: 1537394  

Journal: :The Biochemical journal 2007
Emma Heart Gordon C Yaney Richard F Corkey Vera Schultz Esthere Luc Lihan Liu Jude T Deeney Orian Shirihai Keith Tornheim Peter J S Smith Barbara E Corkey

The present study was undertaken to determine the main metabolic secretory signals generated by the mitochondrial substrate MeS (methyl succinate) compared with glucose in mouse and rat islets and to understand the differences. Glycolysis and mitochondrial metabolism both have key roles in the stimulation of insulin secretion by glucose. Both fuels elicited comparable oscillatory patterns of Ca...

Journal: :Plant physiology 1976
A C Magalhães D B Peters R H Hageman

The effect of various day temperatures on NADH-nitrate reductase, NADH- and NADPH-glutamate dehydrogenases, nitrate, protein and leaf area, measured at intervals during the ontogeny of the first trifoliolate soybean leaf, was determined. At 32.5 C and 25 C, nitrate concentration, nitrate reductase, and NADPH-glutamate dehydrogenase activities increased concurrently with leaf development and the...

Journal: :Fertility and sterility 2004
Tamer M Said Ashok Agarwal Rakesh K Sharma Edward Mascha Suresh C Sikka Anthony J Thomas

OBJECTIVE To measure levels of reactive oxygen species (ROS) including H(2)O(2) and O(2)(.-) generation in infertile men and determine whether sperm quality is correlated with levels of ROS triggered by the exogenous reduced form of beta nicotinamide adenine dinucleotide phosphate (NADPH). DESIGN Prospective study. SETTING Male infertility clinic at a tertiary healthcare center. PATIENT(S...

Journal: :Arteriosclerosis, thrombosis, and vascular biology 2006
Helene Girouard Laibaik Park Josef Anrather Ping Zhou Costantino Iadecola

OBJECTIVE Angiotensin II (Ang II) exerts deleterious effect on the cerebral circulation through production of reactive oxygen species (ROS). However, the enzymatic source of the ROS has not been defined. We tested the hypothesis that Ang II impairs endothelium-dependent responses in the cerebral microcirculation through ROS generated in cerebrovascular cells by the enzyme NADPH oxidase. METHO...

Journal: :American journal of physiology. Heart and circulatory physiology 2009
Anuradha Dhanasekaran Sreedhar Bodiga Stephanie Gruenloh Ying Gao Laurel Dunn John R Falck J Noelle Buonaccorsi Meetha Medhora Elizabeth R Jacobs

20-Hydroxyeicosatetraenoic acid (20-HETE) is an endogenous cytochrome P-450 product present in vascular smooth muscle and uniquely located in the vascular endothelium of pulmonary arteries (PAs). 20-HETE enhances reactive oxygen species (ROS) production of bovine PA endothelial cells (BPAECs) in an NADPH oxidase-dependent manner and is postulated to promote angiogenesis via activation of this p...

Journal: :Hypertension 2002
Jian-Mei Li Nick P Gall David J Grieve Mingyou Chen Ajay M Shah

Increased reactive oxygen species (ROS) production is implicated in the pathophysiology of left ventricular (LV) hypertrophy and heart failure. However, the enzymatic sources of myocardial ROS production are unclear. We examined the expression and activity of phagocyte-type NADPH oxidase in LV myocardium in an experimental guinea pig model of progressive pressure-overload LV hypertrophy. Concom...

Thioredoxins (Trxs) are small ubiquitous oxidoreductase proteins with two redox-active Cys residues in a conserved active site (WCG/PPC) that regulate numerous target proteins via thiol/disulfide exchanges in the cells of prokaryotes and eukaryotes. The isoforms OsTrx23 with a typical active site (WCGPC) and OsTrx20 with an atypical active site (WCTPC) are two  Trx h- type isoforms in rice that ...

Journal: :The Biochemical journal 1965
A FRANCKE

1. A description is given of the course of the reduction of 5-oxodecanoic acid to 5-hydroxydecanoic acid by intact cells of baker's yeast and of the influence of pH on this reduction. As the pH of the medium is decreased the rate of uptake of the keto acid by yeast increases. However, the more the rate of uptake increases the more rapidly the yeast is poisoned by the keto acid or hydroxy acid o...

Journal: :The Biochemical journal 1988
J L Schrimsher P T Wingfield A Bernard R Mattaliano M A Payton

5-Ketofructose reductase [D(-)fructose:(NADP+) 5-oxidoreductase] was purified to homogeneity from Erwinia citreus and demonstrated to catalyse the reversible NADPH-dependent reduction of 5-ketofructose (D-threo-2,5-hexodiulose) to D-fructose. The enzyme appeared as a single species upon analyses by SDS/polyacrylamide-gel electrophoresis and isoelectric focusing with an apparent relative molecul...

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