Homogeneous, recombinant 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Neisseria gonorrhoeae is shown to catalyze the formation of 3-deoxy-D-manno-octulosonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate as determined from (1)H-nuclear magnetic resonance analysis of the product. This enzyme does not catalyze the condensation of D-erythrose 4-phosphate and phosphoen...

1. Abstract 2. Introduction 3. Biogenesis of O-specific lipopolysaccharides 3.1. Initiation reaction 3.1.1. Polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transferases 3.1.2. Polyisoprenyl-phosphate hexose-1-phosphate transferases 3.2. Elongation/translocation/polymerization 3.2.1. Wzy/Wzx-dependent pathway 3.2.2. ABC transporter pathway 3.2.3. Synthase pathway 3.3. Ligation reaction 3....

In Escherichia coli, 1-deoxy-D-xylulose (or its 5-phosphate, DXP) is the biosynthetic precursor to isopentenyl diphosphate [Broers, S. T. J. (1994) Dissertation (Eidgenössische Technische Hochschule, Zürich)], thiamin, and pyridoxol [Himmeldirk, K., Kennedy, I. A., Hill, R. E., Sayer, B. G. & Spenser, I. D. (1996) Chem. Commun. 1187-1188]. Here we show that an open reading frame at 9 min on the...

The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14- to 20-fold over ...

DAHP2 synthase (EC 4.1.2.15) is the first enzyme of the shikimate pathway that leads to the biosynthesis of the aromatic amino acids and derived secondary metabolites. The enzyme is induced by glyphosate (6), wounding (1, 4), and fungal elicitor (4, 5). Previously, a cDNA encoding DAHP synthase, which we call shkA, was cloned and sequenced from potato cells grown in suspension culture (2); that...