P34 and P37 are two previously identified RNA binding proteins in the flagellate protozoan Trypanosoma brucei. RNA interference studies have determined that the proteins are involved in and essential for ribosome biogenesis. The proteins interact with the 5S rRNA with nearly identical binding characteristics. We have shown that this interaction is achieved mainly through the LoopA region of the...

In this paper, we describe ongoing research in three DL projects that build upon a common foundation – the 5S DL framework. In each project, we extend the 5S framework to provide specifications for a particular type of DL service and/or system – finally, moving towards a DL reference model. In the first project, we are working on formalizing content-based image retrieval services in a DL. In th...

A structure has been obtained for the loop E region of the 5S rRNA from Spinacia oleracia chloroplast ribosomes using residual dipolar coupling data as well as NOE, J coupling and chemical shift information. Even though the loop E sequence of this chloroplast 5S rRNA differs from that of Escherichia coli loop E at approximately 40% of its positions, its conformation is remarkably similar to tha...

We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed i...

A precursor of 5S ribosomal ribonucleic acid (rRNA) from Bacillus subtilis was cleaved by ribonuclease (RNase) M5 in cell-free extracts from B. subtilis to yield the mature 5S rRNA plus RNA fragments derived from both termini of the precursor. The released, mature 5S rRNA was stable in these extracts; however, as occurred in vivo, the precursor-specific fragments were rapidly and completely des...

A group of 96 nondefective children in Grades 4 through 9 who were referred to a child psychiatric clinic were compared with a group of 120 public school children of similar intellectual and educational level in terms of their response to a Taffel-type verbal conditioning procedure. Half of the children in each group were experimental 5s, to whom E said "good" following their use of a first-per...

BACKGROUND The 5S management method (where 5S stands for sort, set in order, shine, standardize, and sustain) was originally implemented by manufacturing enterprises in Japan. It was then introduced to the manufacturing sector in the West and eventually applied to the health sector for organizing and standardizing the workplace. 5S has recently received attention as a potential solution for imp...

During the last several years an opportunity for B s meson studies at the e e colliders running at the Υ(5S) resonance has been extensively explored. The first evidence for B s production at the Υ(5S) was found by the CLEO collaboration [1, 2] using a dataset of 0.42 fb collected in 2003. This study indicated that practicalB s measurements at the Υ(5S) are possible with a dataset of at least 20...

The objective of this study was to understand the perception of a multidisciplinary health team regarding the implementation of a quality management took at a teaching hospital. The setting of this qualitative study was the University Hospital at University of São Paulo (HU-USP). Participants were seven workers from the Committee for the Implementation of the 5S Program. Data collection was per...

Bacillus stearothermophilus 50S ribosomal subunits were reconstituted in vitro using artificial 5S RNA molecules constructed by combining parts of major and minor type (Raué et al. (1976) Europ. J. Biochem. 68, 169-176) B. licheniformis 5S RNA. The artificial 5S RNA molecules carry defined disturbances (A.C juxtapositions and extra G.U pairs) in the base pairing between the 5'- and 3'-terminal ...