ObjectivesMolecular assays on nasopharyngeal swabs remain the cornerstone of COVID-19 diagnostics. The high technicalities sampling and molecular assays, as well scarce resources reagents, limit our testing capabilities. Several strategies failed, to date, fully alleviate this process (e.g. saliva or antigen samples). We assessed clinical performances SARS-CoV-2 nucleocapsid (N-antigen) ELISA d...

The O-antigen component of the lipopolysaccharide (LPS) represents a population of polysaccharide molecules with nonrandom (modal) chain length distribution. The number of the repeat O units in each individual O-antigen polymer depends on the Wzz chain length regulator, an inner membrane protein belonging to the polysaccharide copolymerase (PCP) family. Different Wzz proteins confer vastly diff...

We investigated whether HIV-1 antigen-specific CD4(+) T cells expressed the viral coreceptor CCR5 during primary HIV-1 infection (PHI). In the peripheral blood of subjects with very early PHI (< 22 days after onset of symptoms), there was a 10- to 20-fold increase in the proportion of highly activated (CD38(+++)) and proliferating (Ki-67(+)) CD4(+) T cells that expressed CCR5(+), and were mostl...

AIM To compare the Ki-67 labelling index determined using the enhanced peroxidase one step (EPOS) method with that using the standard ABC technique in pituitary tumours. METHODS Adjacent sections were immunostained using the EPOS and ABC techniques with the same Ki-67 antibody and same antigen retrieval method. RESULTS The labelling index measurements with the EPOS Ki-67 antibody were signi...

Chromatin assembly factor 1 (CAF-1) deposits histones during DNA synthesis. The p150 subunit of human CAF-1 contains an N-terminal domain (p150N) that is dispensable for histone deposition but promotes the localization of specific loci (nucleolar-associated domains [NADs]) and proteins to the nucleolus during interphase. One of the p150N-regulated proteins is proliferation antigen Ki-67, whose ...

A rapid dipstick test for scrub typhus was prospectively evaluated in Chiangrai, northern Thailand. Sera from 162 patients with fever of unclear etiology were tested by a dot blot immunoassay using two different antigen concentrations. Dipsticks coated with lower concentration of antigen lacked sensitivity compared with the indirect immunoperoxidase test. Dipsticks with higher antigen concentra...

background : basal cell carcinoma is the most common form of skin cancer in men. increased marker expressions of apoptosis and cell proliferation have been found in the majority of basal cell carcinomas. our purpose was to determine whether any correlation between immunohistochemical expressions of p53 and ki-67 in different histopathological variants of basal cell carcinoma and patient’s age, ...