A novel, strictly anaerobic, cadaverine-oxidizing, defined coculture was isolated from an anoxic freshwater sediment sample. The coculture oxidized cadaverine (1,5-diaminopentane) with sulfate as the electron acceptor. The sulfate-reducing partner could be replaced by a hydrogenotrophic methanogenic partner. The defined coculture fermented cadaverine to acetate, butyrate, and glutarate plus sul...

Statins are mainly used as the inhibitors of enzyme 3-hydroxy-3 methyl glutaryl coenzyme A (HMG COA) reductase which is responsible for the synthesis of cholesterol. Apart from the cholesterol reducing activity they also facilitate as antiinflammatory and cardio-protective agents. Solubility is one of the main parameter for any drug to achieve the expected therapeutic effects. Since Simvastatin...

The enzymes catalysing the initial stage of the 8-oxidation of fatty acids, the acyl-CoA synthetases, have been classified into four groups based on specificity. These are: the short-chain (acetyl-CoA synthetase; EC 6.2.1 .l), medium-chain (butyryl-CoA synthetase; EC 6.2.1.2) and the long-chain fatty acyl-CoA synthetase (acyl-CoA synthetase; EC 6.2.1.3), which are ATP-dependent and follow the r...

Objective(s): As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. Materials and Meth...

The screening of a rat liver cDNA library with a hamster probe ACKNOWLEDGEMENTS that encompasses the nucleotides coding for the hamster enzyme . . . c . n T n w *~< J W C c A, , , • ,A A TAXI A A . U . 1 We are grateful to Drs. J.D.McGarry and V.Esser for providing (1), yielded a cDNA encoding the rat cytosolic ° yu 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase. t h e r a t h v e r c...

Statins inhibit hepatic 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) reductase with consequent suppression of cholesterol biosynthesis. The advent of these drugs has significantly impacted the treatment of cardiovascular disease with well documented benefit in primary and secondary prevention1-3. However, several clinical and basic science investigations suggested that the beneficial effects...

Downey, Ronald J. (University of Nebraska, Lincoln), Carl E. Georgi, and Walter E. Militzer. Electron transport particles from Bacillus stearothermophilus. J. Bacteriol. 83:1140-1146. 1962-Electron-transport particles (ETP) have been isolated from Bacillus stearothermophilus. They are capable of oxidizing such substrates as succinate, malate, diphosphopyridine nucleotide, p-phenylenediamine, an...

Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (EC 4.1.3.5) was purified from ox liver, and obtained essentially free from 3-oxoacyl-CoA thiolases. The kinetic behaviour, like that of the synthases from chicken liver and yeast, is compatible with a reaction pathway involving condensation of an acetyl-enzyme with acetoacetyl-CoA. The Km for acetoacetyl-CoA, less than 1 micronM at pH 7.8, ...

Adriamycin inhibits the succinoxidase system and the NADH-oxidase system. Both of the intact mitocondrial enzymes and the pentane-extracted preparations are inhibited. The inhibition can be prevented by a molar ratio of coenzyme to adriamycin of 3:1 for coenzyme Q10 (ubiquinone), 5:1 for coenzyme Q7, and 5:1 for coenzyme Q4. Prevention of inhibition was observed in the decreasing order of coenz...

AIM P wave dispersion (PWD) has been shown to be a noninvasive predictor for the development of atrial fibrillation (AF). Atorvastatin is a 3 hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase inhibitor that lowers blood cholesterol levels. The beneficial effect of atorvastatin on atrial arrhythmias is controversial. Aim of this study was to investigate the effect of atorvastatin treatment o...