biochemical polymorphism study is a method of determination of genetic variation. this variability could be a basis for selection and subsequent genetic improvement in farm animals. the polymorphism in the intron 1 of chicken growth hormone (cgh) gene was investigated in the iranian native fowls by using polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) method. the...

BACKGROUND:Abortion is one of the most important factorsreducing lambing rate and consequently profitability of sheepfarms. In addition to financial losses, it is also important from azoonotic point of view. OBJECTIVES: The aim of this study wasto investigate bacterial abortifacient agents in an outbreak ofabortion occurring in Afshari sheep in the northwest of Zanjanprovince. METHODS:Vaginal s...

toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. toxoplasma gondii-derived heat shock protein 70 (hsp70) may play an important role in the virulence of toxoplasma gondii (t. gondii). in the present study, t. gondii hsp70 was amplified by polymerase chain reaction (pcr) from the dna of the t. gondiitachyzoite rh strain through the use o...

Prostate-specific membrane antigen (PSMA) is a protein that is expressed predominantly in normal prostate epithelial cells and in most adenocarcinomas of the prostate (Cap) and in virtually all Cap metastases. In this article we describe the cloning of a 2-kb human genomic DNA fragment containing the 5' upstream untranslated region of the PSMA gene and present evidence that it provides promoter...

The hemolymph chymotrypsin inhibitor b1 (CIb1) of silkworm, Bombyx mori, plays an important role in innate immunity. In order to study its encoding gene CIb1, five heterogeneous promoter fragments of 844 bp, 682 bp, 516 bp, 312 bp and 82 bp in length were cloned from genomic DNA of the p50 silkworm strain. Characterization of the CIb1 promoter was performed in vitro using the firefly luciferase...

This unit describes methods for recovering and purifying DNA restriction fragments from agarose gels. The first basic protocol describes electroelution of the fragment of interest from standard agarose gels using buffer-filled dialysis bags, followed by concentration and purification using an Elutip column. This approach can be used effectively for fragments of all sizes from 50 to 20,000 bp. E...

giardia is one of the most common human parasites and causes a lengthly course of nonbacterial diarrhea. disease outbreaks due to giardia infection are often attributed to contaminated water supplies. a major problem associated with detection for this organism is the lack of sensitive and reliable methods. pcr has the potential to address many of the limitations.we have performed a pcr-based me...

Casein is a family of milk proteins that exists in several molecular forms and is the main protein present inthe bovine milk. The B variant of bovine k-casein is reported to be favorable for quality and quantity of cheese derived from milk and considered to be included in breeding strategies of dairy cattle. Genotypes of72 Russian Black Pied and 80 Red Pied cows were determined for ...