Amphipols (APols) are specially designed amphipathic polymers that stabilize membrane proteins (MPs) in aqueous solutions in the absence of detergent. A8-35, a polyacrylate-based APol, has been grafted with an oligodeoxynucleotide (ODN). The synthesis, purification and properties of the resulting 'OligAPol' have been investigated. Grafting was performed by reacting an ODN carrying an amine-term...

Measurement of the photofragment velocity and angular distributions from the photodissociation of N,N-dimethylformamide at 193 nm in its pnbp* absorption evidences three competing dissociation channels: HCON~CH3!2→HCO(X̃ A8)1N~CH3!2(X̃ B1); HCO(X̃ A8)1N~CH3!2(Ã A1); and HCONCH31CH3. ~H atom eliminations are not probed.! These products are formed in a ratio of 0.1560.04:0.4960.09:0.3660.07, determi...

Macaque retinae were immunostained with monoclonal antibodies directed against the protein synaptotagmin-2 (Syt2). Syt2 was localized in a population of small-field amacrine cells, whose cell bodies formed a regular mosaic within the inner nuclear layer, indicating they represent a single amacrine cell type. The labeled amacrine cells had a bistratified appearance with a dense dendritic plexus ...

We report the first French case of percutaneous balloon pulmonary angioplasty (BPA) in a 78-year-old patient with chronic thromboembolic pulmonary hypertension (CTEPH). The patient had a history of acute pulmonary embolism in 2004 with recurrence in 2011. At the end of 2012, he complained of persistent dyspnoea and CTEPH was diagnosed on lung radionuclide perfusion scan, contrast chest computed...

Radiofrequency ablation (RFA) is commonly used as a treatment for small hepatocellular carcinoma (HCC). Although several complications such as intraperitoneal bleeding are often observed after RFA, hepatic arterioportal fistula (APF) is a less frequently occurring complication. In this study, we describe two cases of APF caused by RFA, which was successfully occluded by an interventional approa...

Ultraviolet photoelectron spectra of CH3O , CH3CH2O , (CH3)2CHO , (CH3)3CO , as well as CD3O 2 and CD3CD2O 2 are presented, providing improved electron affinities and new information on vibronic coupling in the corresponding neutral radicals. Jahn-Teller vibronic transitions are assigned to e vibrational modes of X̃ E CH3O. The excitation energy of the à A8 state of CH3CH2O with respect to the X̃...

Preeclampsia is the major pregnancy-induced hypertensive disorder. It modifies the expression profile of placental genes, including several serine protease inhibitors (SERPINs). The objective of this study was to perform a systematic expression analysis of these genes in normal and pathological placentas and to pinpoint epigenetic alterations inside their promoter regions. Expression of 18 plac...

Complement is an ancient part of the innate immune system that plays a pivotal role in protection against invading pathogens and helps to clear apoptotic and necrotic cells. Upon complement activation, a cascade of proteolytic events generates the complement effectors, including the anaphylatoxins C3a and C5a. Signalling through their cognate G-protein coupled receptors, C3aR and C5aR, leads to...

Adrenergic agents modulate the activity of midbrain ventral tegmental area (VTA) neurons. However, the sources of noradrenergic and adrenergic inputs are not well characterized. Immunostaining for dopamine beta-hydroxylase revealed fibers within dopamine (DA) neuron areas, with the highest density in the retrorubral field (A8 cell group), followed by the VTA (A10 cell group), and very few fiber...

OBJECTIVES General thoracic surgeons must be familiar with anatomical variation in the pulmonary vessels and bronchi. Here, we analyzed the bronchovascular pattern of the right middle lobe (RML) and right lower lobe (RLL) of the lung using three-dimensional CT angiography and bronchography (3DCTAB). METHODS We reviewed the anatomical patterns of the pulmonary vessels and bronchi in 270 patien...