The amount of the third component of complement (C3) bound to red cells of patients with the cold agglutinin syndrome was determined by a quantitative assay, measuring the fixation of the first component of complement by anti-C3. Abrupt reduction in the serum concentration of cold agglutinin by plasmapheresis markedly decreased the hemolytic rate, but the amount of C3 bound to circulating cells...

BACKGROUND The aim of this study was to examine if gradual withdrawal of remifentanil infusion prevented opioid-induced hyperalgesia (OIH) as opposed to abrupt withdrawal. OIH duration was also evaluated. METHODS Nineteen volunteers were enrolled in this randomized, double-blinded, placebo-controlled, crossover study. All went through three sessions: abrupt or gradual withdrawal of remifentan...

Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) ...

Experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. Ejaculates were collected from four rams. In each session, second ejaculates (n=4) were collected in a tube containing 1 mL coating buffers which were prepared by 10, 15 or 20% egg yolk plus Tris-fructose. Samples were pooled, centrifuged and the supernatant removed in the labora...

Cold shock proteins are nucleic acid-binding proteins. They are well conserved in bacteria, animals as well as plants. Prokaryotic CSPs possess a single cold shock domain while animal CSPs are flanked by Nand C-terminal domains. Eukaryotic CSPs are commonly named Y-box proteins. Interestingly, the plants CSPs contain a C-terminal domain in addition to their N-terminal CSD. The CSPs have been re...

From the physical point of view a shock wave is defined by an abrupt, nearly discontinuous change in pressure and by having a velocity that is higher than the speed of sound in the medium it propagates [1]. A typical pressure profile of a focused shockwave used for therapeutic purposes is shown in fig. 1. Generally a shock wave can be described as a single pulse with a wide frequency range (fro...

DNA binding protein A (DbpA) is a member of the human cold shock domain-containing protein superfamily, with known functions in cell proliferation, differentiation, and stress responses. DbpA mediates tight junction-associated activities in tubular epithelial cells, but the function of DbpA in mesangial cells is unknown. Here, we found DbpA protein expression restricted to vascular smooth muscl...

When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 degrees C or 15 degrees C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. The rate of CS7.4 production reached 13% of total protein synthesis within 1-1.5 hr after a shift to 10 degrees C and subsequently dropped to a lower basal level. Regulation of CS7.4 express...