Microbial terminal oxidases are usually associated with particulate fractions in the cell. These fractions, however, are difficult to resolve into smaller active molecules that are suitable for enzymatic studies in vitro. This fact led Rittenberg (1) to propose that these particles are surrounded by a special membrane. The present paper will report a method of “solubilization”’ of particulate o...

Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. trop...

We investigated the sequence and the genetic context of the erm(T) gene in six inducible erythromycin-resistant Streptococcus gallolyticus subsp. pasteurianus (formerly S. bovis biotype II.2) isolates. In all isolates, the erm(T) genes were flanked by two IS1216V-like elements with the same polarity and were found to be inserted in the chromosome.

First Isolation of Streptococcus gallolyticus subsp. pasteurianus from a Korean Patient with Severe Septic Shock Seri Jeong, Ji Yeon Park, Sang Hoon Han, Yangsoon Lee, Dongeun Yong, Kyungwon Lee, Yunsop Chong Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Department of Laboratory Me...

Here we report the 3.7-Mb draft genome sequence of Acetobacter tropicalis NBRC16470(T), which can produce optically pure d-glyceric acid (d-GA; 99% enantiomeric excess) from raw glycerol feedstock derived from biodiesel fuel production processes.

Nowadays, vinegar is industrially produced by mesophilic acetic acid bacteria (AAB). However, temperature fluctuation during acetous fermentation is inevitable, and may cause process disturbances. This can be mostly avoided using thermo-tolerant AAB. The main purpose of the present study was to isolate thermo-tolerant AAB. Fermentation performances were then evaluated. Twenty-eight different is...