Search for safe antioxidants and novel nutraceuticals urged to evaluate the antioxidant, anti-acetylcholine esterase anti-lipoxygenase activity of various leaf extracts Conocarpus lancifolius. Extraction was optimized from freeze dried plant quenched with liquid nitrogen using water, ethanol, methanol, hexane, ethyl acetate chloroform. Maximum extract yield, total phenolic contents flavonoid we...

To determine the effects of acetylcholine on the coronary bed in the baboon and whether the effects preceded or followed the action of acetylcholine on ventricular function, eight adult baboons (Papio anubis) were instrumented to measure left ventricular (LV) and mean arterial pressures, LV dP/dt, regional myocardial function, and coronary blood flow. Acetylcholine was injected locally through ...

Acetylcholine, the parasympathetic neurotransmitter, shortens the action potential duration of cat atrial muscle cells, but not ventricular muscle cells. In mammalian species, atrial tissue receives a richer cholinergic nerve supply than ventricular tissue. To determine whether chronic withdrawal of cholinergic tone might influence the subsequent response of these tissues to cholinergic stimula...

BACKGROUND The authors investigated the extent and cellular mechanisms by which the intravenous anesthetic ketamine alters acetylcholine-induced contraction in pulmonary veins (PVs). They tested the hypothesis that ketamine inhibits acetylcholine contraction in PVs. METHODS Canine PV rings with endothelium (E+) and without endothelium (E-) were isolated for measurement of isometric tension. T...

Studies of the binding of [3H]acetylcholine to receptor-rich membranes of Torpedo californica electric organ under conditions that normally lead to a state of equilibrium did not give rise to equilibrium binding curves. Instead, the acetylcholine receptor was found to develop very long lived metastable states resulting in hysteresis in binding. Under conditions where the concentration of free [...

Crude extracts and three isolated alkaloids from Erythrina mulungu plants have shown anxiolytic effects in different animal models. We investigated whether these alkaloids could affect nicotinic acetylcholine receptors and if they are selective for different central nervous system (CNS) subtypes. Screening experiments were performed using a single concentration of the alkaloid co-applied with a...

Cataplexy in the narcoleptic canine has been shown to increase after local administration of carbachol into the pontine reticular formation. Rapid eye movement sleep has also been shown to increase after local administration of carbachol in the pontine reticular formation, and furthermore, acetylcholine release in the pontine tegmentum was found to increase during rapid eye movement sleep in ra...

In various skeletal muscles, the mean density of acetylcholine receptors in the muscle postsynaptic membrane is constant at about 8700 per mum(2), even though the overall size of an endplate ranged from 400 mum(2) to 1300 mum(2) in these muscles. This measurement was by electron microscope autoradiography of alpha-[(3)H]bungarotoxin binding sites; only one-half of these, however, appear to be t...

background: food-born botulism is caused by ingestion of contaminated food with clostridium botulinum neurotoxin. case: in food-born botulism, due to acetylcholine release blockade, neuromuscular signs and symptoms are common, but in this case report we present a food-born botulism with papiledema and venous sinus thrombosis. conclusion: papiledema and fever could not rule out the diagnosis of ...