The feasibility of using the Hale stain to identify cellular sialic acid-containing mucins by electron microscopy was investigated. Three kinds of mouse ascites tumor cells were fixed in neutral buffered formalin, exposed to fresh colloidal ferric oxide, treated with potassium ferrocyanide, imbedded in Selectron, and sectioned for electron microscopy. Additional staining with uranyl acetate and...

introduction: ursolic acid (ua) is a lipophilic triterpenoid compound, found in large amounts in apple peel. anabolic effects of ua on the skeletal muscle and the role of this tissue as a key regulator of systematic aging aroused this question in mind whether ua might amend skeletal muscle performances such as myoglobin expression and also whether it switches skeletal muscle fibers from glycoly...

Materials and Methods Formalin-fixed paraffin-embedded lung SqCC (n=137) and LADC (n=60) tissue microarrays were acquired. DSG3/p40 + Napsin A double stain cocktail (Biocare Medical, Concord, CA), TTF-1 [SPT24] and TTF-1 [8G7G3/1] antibody titers were optimized for IHC. SPT24 was optimally titered at 1:1200. The double stain antibody cocktail was detected by using a polymer double stain detecti...

Lymph nodes from guinea pigs inoculated with Mycobacterium tuberculosis were fixed in buffered formalin, then treated for the recommended times in Gooding and Stewart's fluid, EDTA, aqueous nitric acid, von Ebner's fluid, and rapid decalcifier (RDC). The blocks were processed to paraffin wax and sections were stained by the Ziehl-Neelsen technique. Only in sections of the blocks treated with RD...

Tuberculosis (TB) is a serious lung infection caused by the bacterium Mycobacterium tuberculosis ( M. ) highly contagious, and can be spread through air from an infected person to others. The aim of this article was evaluate immunological role CD4 CD20 in patients with multi-drug resistant (MDRTB). A total 182 individuals suspected admitted center AL-Najaf City, Iraq. sputum acid-fast stain per...

A 42-year-old male presented in June of 2011 with nocturnal fevers, night sweats, an 8-kg weight loss, and a cutaneous right chest wall mass. In March of 2013, a computed tomographic scan of the thorax showed a 54 × 18 × 26-mm right lower lobe mass with peripheral calcifications, and in May of 2013, he was admitted for a segmental lobectomy, in which histologic examination of the pulmonary tiss...

Stool specimens taken from 50 children with malignancy and from 92 healthy children were investigated for intestinal parasites, using the modified formol ethyl acetate concentration method, and native-lugol, trichrome and Kinyoun acid-fast stain methods. Thirty-eight (76.0%) of the 50 patients had lymphoma or leukemia and were considered immunosuppressed. Several different parasites were found ...

The indolent and nonspecific presentations of tuberculous arthritis and pyomyositis render their diagnosis a great challenge for clinicians. We describe a 78-year-old man with swelling and pain of right knee for 6 months. The patient was empirically treated for tuberculosis because positive enzyme-linked immunospot assay for interferon-γ of blood was reported at 2 days after aspiration of synov...