Histone H1 is highly phosphorylated in mitotic HeLa cells, but is quickly dephosphorylated in vivo at the end of mitosis and in vitro following cell lysis. We show here that okadaic acid and microcystin-LR block the in vitro dephosphorylation of H1 and that they do so directly by inhibiting the histone H1 phosphatase rather than by some indirect mechanism. The concentrations of microcystin and ...

THE distribution of acid phosphatase in the central nervous system as demonstrated by histochemical methods has been investigated by LANDOW, KABAT and NEWMAN (1942), WISLOCKI and DEMPSEY (1948), and by SHIMIZU (1950) in comparative studies that included mice, rats, guinea pigs, rabbits, cats and chickens. Numerous papers deal with the cytological distribution of acid phosphatase in selected reg...

Periodic acid-Schiff staining, acid phosphatase localization, and yellow autofluorescence have been correlated with the PAS-body of Gonyaulax polyedra for the first time. PAS- staining and acid phosphatase activity are both correlated with the PAS-body of Gonyaulax tamarensis. These results that the PAS-body of these marine dinoflagellate algae functions in subcellular digestion.

To evaluate phosphorus (P) availability and the role of microorganisms in P dynamics barley rhizosphere, we constructed a rhizobox using two arable Andosols under different fertilization management regimens cultivated (Hordeum vulgare L. cv. Minorimugi) for 5 weeks. The phosphatase-labile pool organic (Po) was assessed phosphatase-addition approach combination with chemical extraction Po from s...

Acid phosphatase was purified from human prostatic tissue and from seminal plasma. Antisera to antigens from both sources were raised in rabbits. These antisera have been used for immunohistochemical localization of the respective antigens in the prostates of neonatal, infantile, prepubertal, and adult individuals. Immunoreactivity of the prostatic epithelium with the seminal fluid-derived anti...

Acid phosphatase and ubiquinone reactions have a different distribution in the lens epithelium of the normal rabbit. Acid phosphatase is more concentrated in the germinative area than the central area, and ubiquinone is quite evenly distributed in the entire lens epithelium. Exposure to a cataracto genie dose of x-ray causes a greater decrease in the acid phosphatase in the germinative zone tha...

Multiple forms of hydrolytic enzymes were demonstrated in extracts of healthy bean leaves (Phascolus vulgaris L.) and bean leaves infected with the halo blight organism [Pseudomonas phaseolicola (Burkh.) Dowson] by polyacrylamide disc electrophoresis. Bean leaves contained up to 4 acid phosphatase bands, 9 esterase bands active towards alpha-naphthyl acetate, and 7 esterase bands towards alpha-...

During the past three ,years serum acid and alkaline phosphatase deterlninations have been carried out by Barringer and Woodard (z) , Gutman and Gutman and their co-workers (2-4, io ) , Huggins (7-9), ctc., as a means of facilitating early rccognition of metastatic bone lcsions from carcinoma of the prostate. In recent publications (5, 6) we have pointed out that, in our opinion, serum acid pho...

A correlation was made between the urinary excretion of GOT, GPT, LDH, HBD, and acid and alkaline phosphatase and their serum activity in 18 suspected cases of myocardial infarction. The diagnoses were made by a cardiologist without knowledge of the enzyme assays. The study included 2 cases of nontransmural infarction; 5 cases that had not sustained an infarction served as controls. Elevations ...

The inhibitory/repressive effect of the final reaction product (inorganic o-phosphate) and the inducing effect of a substrate (β-glycerophosphate) on the phosphatase activity and synthesis in a salt lake sediment were studied. The determinations have been differentially carried out, for acid, neutral and alkaline phosphatase, and in reaction mixtures without buffering. The acid phosphatase acti...