Male infertility can be treated by surgical procedures (e.g., varicocelectomy) or by administration of drugs if causal factors (e.g., seminal tract infections) are detected. In more severe cases, methods of assisted fertilization often have to be applied, but even these have only a limited success rate. Recent studies have demonstrated that disturbances of sperm DNA integrity (determined by the...

A fluorescence quenching method was used to determine the equilibrium binding constants for the association of acridine, acridine orange, and acridine yellow G to humic acid. The fluorescence of each polycyclic aromatic nitrogen heterocycle (PANH) was monitored as aliquots of humic acid were added, and a Stern-Volmer plot was produced in which the slope is the equilibrium constant of the bindin...

Background: There is a rapidly growing trend in the consumption of herbal remedies in the developing countries. The Phoenix dactylifera date palm pollen (DPP) is used in the traditional medicine for male infertility. The aim of this study was to determine the effects of orally administered DPP on the reproductive system of adult male rats. Methods: Fifty Sprague-Dawley rats were maintained acco...

background: there are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and dna integrity. objective: to determine the effects of ibuprofen on sperm parameters, chromatin condensation and dna integrity of mice. materials and methods: in this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control (...

Treatment of cultured pancreatic B-cells (HIT-T15 and RINm5F) with the diabetogenic drug streptozotocin resulted in a significant increase in the number of cells that became detached from the substrate during a subsequent culture period. Examination of the detached cells by fluorescence microscopy after staining with acridine orange or by electron microscopy revealed evidence of chromatin conde...

background: the aim of present study is to evaluate the effect of polyvinyl pyrrolidone (pvp) routinly used during icsi procedure on sperm membrane integrity, and sperm chromatin status. materials and methods: this study was carried out on 21 semen samples from the infertile men referring to isfahan fertility and infertility center. the processed semen samples were divided into two portions. on...

background: the particles in the range of 1-100 nm are called nanoparticles56t. gold nanoparticle is one of the most important metal nanoparticles with wide usage56t.objective: 56tthis study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice56t.materials and methods: 56tin this experimental study, 72 male bulb-c mice were divided into 9 groups inc...

Selenium can have cancer chemopreventive activity, although the mechanism of action has not been well defined. Selenazolidine-4-(R)-carboxylic acids (SCAs) were devised as prodrugs of L-selenocysteine, to provide selenium in a form and at a concentration commensurate with cancer chemopreventive activity. In the present study, a series of selenazolidines has been evaluated in the Salmonella typh...

BACKGROUND Human sperm DNA damage may adversely affect reproductive outcomes, and the spermatozoa of infertile men possess substantially more DNA damage than that of fertile men. To date, there is no available treatment for men with high levels of sperm DNA damage. The objective of this study was to examine the effect of varicocelectomy on sperm DNA denaturation (DD, an index of sperm DNA damag...