The mitochondrial rotenone-sensitive NADH:ubiquinone oxidoreductase (complex I) comprises more than 30 subunits, the majority of which are encoded by the nucleus. In Chlamydomonas reinhardtii, only five components of complex I are coded for by mitochondrial genes. Three mutants deprived of complex I activity and displaying slow growth in the dark were isolated after mutagenic treatment with acr...

This distance between a base next to the anticodon of tRNA and the 3' CpCpA terminus of the molecule has been estimated by singlet-singlet energy transfer experiments. The energy donor was the Y base of unknown structure found in yeast tRNA(phe). Three different energy acceptors were used: acriflavine, proflavinyl acetic acid hydrazide, and 9-hydrazino acridine. These were attached to the perio...

upon by me the next morning-. Amputation by the modified circular method was performed a few inches below the shoulder joint, and a drainage tube inserted. The following are brief notes of this case after operation:? 28-7-26. Temperature 102.5 ?F in the morning, and 103?F in the evening. One pint of saline given intravenously. Blood films examined for malarial parasites with negative result. 10...

Cytophotometric DNA determination was performed on 8 glioblastomas, 4 astrocytomas, 2 oligoden drogliomas, and 4 meningiomas. Tumor cells were separated from a paraffin section and stained for DNA using the acriflavine-Feulgen nuclear reaction. Nonspecific dye-binding was blocked by azocarmin G staining. All the specimens were postirradiated to eliminate primary fluorescence and to stabilize th...

BACKGROUND The review aimed to compare the effectiveness, safety and acceptability of vasectomy techniques for male sterilization. METHODS We searched five computerized databases and reference lists of relevant articles and book chapters for randomized controlled trials (RCTs) and controlled clinical trials (CCTs) comparing vasectomy techniques. Two reviewers independently extracted data from...

In Neisseria gonorrhoeae and Neisseria meningitidis, we identified a gene that would encode a protein highly similar to NorM of Vibrio parahaemolyticus (Y. Morita et al., Antimicrob. Agents Chemother. 42:1778-1782, 1998). A nonpolar insertional mutation in either the gonococcal or meningococcal norM gene resulted in increased bacterial sensitivity to compounds harboring a quaternary ammonium on...