The preparation of cytoplasmic membranes from suspensions of Staphylococcus aureus lysed by an enzyme recently isolated in these laboratories is described. These membranes contained: protein, 34.4%; ribonucleic acid, 6.6%; lipids, 34.5%; and total phosphorus, 1.4%. Such membranes exhibited adenosine 5'-triphosphatase (E.C. 3.6.1.3) activity, liberating orthophosphate at an initial rate of 0.53 ...

Photophosphorylation, the light dependent formation of adenosine triphosphate from adenosine diphosphate and inorganic phosphate, has been shown to be essentially an irreversible reaction (1). This conclusion was based mainly on the absence of exchange between adenosine triphosphate and inorganic phosphate, or between adenosine diphosphate and adenosine triphosphate (2, 3). In these respects, p...

The authors have studied the acute effects of the intravenous and intia-arterial injection of the sodium salt of adenosine triphosphate (ATP) in man. Rapid injection causes a frightening sensation in the chest and hYperpnea. The changes found agree with those reported in cats and are consistent with the concept that adenosine triphosphate is a vasodilator in somatic structures but causes an inc...

The pool of chemical energy in an organism represented by high-energy compounds can be assessed by means of adenosine triphosphate (ATP) determination in whole blood and tissues. The elegant manner for the determination of adenosine phosphates (ATP, ADP, AMP) in a single assay is offered by the technique of capillary zone electrophoresis. For this purpose, the BioFocus 3000 Capillary Electropho...

The authors have studied the acute effects of the intravenous and intia-arterial injection of the sodium salt of adenosine triphosphate (ATP) in man. Rapid injection causes a frightening sensation in the chest and hYperpnea. The changes found agree with those reported in cats and are consistent with the concept that adenosine triphosphate is a vasodilator in somatic structures but causes an inc...

The participation of pyrophosphate-dependent phosphofructokinase (PPi-PFK) in plant glycolysis was examined using extracts from pea seeds (Pisum sativum L. cv Alaska). Glycolysis starting with fructose 6-phosphate was measured under aerobic conditions as the accumulation of pyruvate. Pyruvate accumulated in a medium containing PPi and adenosine diphosphate at about two-thirds of the rate in a m...

Although cardioplegia reduces myocardial metabolism during ischemia, adenosine triphosphate (ATP) depletion occurs, which may contribute to poor functional recovery after reperfusion. Augmenting myocardial adenosine during ischemia is successful in improving ATP repletion and myocardial recovery following ischemia. If adenosine is an important determinant of ischemic tolerance, then depletion o...

Rab GTPases are essential for vesicular transport, whereas adenosine triphosphate (ATP) is the most important and versatile of the activated carriers in the cell. But there are little reports to clarify the connection between ATP and Rab GTPases. A cDNA clone (Rab14) from Bombyx mori was expressed in Escherichia coli as a glutathione S-transferase fusion protein and purified. The protein bound ...

SLEEP DEPRIVATION HAS LONG BEEN USED AS A TOOL TO STUDY THE FUNCTION OF SLEEP. It has been well documented that sleep deprivation results in significant consequences, including daytime sleepiness and substantial functional impairment.1 Numerous studies have shown that slow wave sleep (SWS) increases during recovery sleep after partial or total sleep deprivation. The amount of both conventionall...

The hydrolysis of adenosine triphosphate (ATP) during actin (de)polymerization consists of two subprocesses, ATP cleavage and phosphate (P_{i}) release, which involve three nucleotide states of each actin protomer. A new theoretical model that explicitly incorporates these different subprocesses and states is introduced and compared with recent experimental data for actin depolymerization. Thes...