The activation of stretch-activated calcium channels (SACs) in keratocytes can induce spatially coordinated increases in traction stress that promote protrusion at the cell front, while simultaneously inducing retraction at the rear. To investigate how this occurs, we correlated calcium-induced changes in traction stress with alterations in cell speed and shape. Cyclic changes in these paramete...

The in vivo effects on the retinal adhesive force of injecting metabolically active agents into the subretinal space of rabbits were studied. Small retinal detachments (blebs) were made in living Dutch rabbit eyes by injecting experimental solutions into the subretinal space with a micropipette. A second micropipette, inserted into the same bleb, measured fluid pressure using a resistance servo...

BACKGROUND The dilution and rapid elimination of topically applied drugs due to the flushing action of saliva is a major difficulty in the effort to eradicate infections of oral cavity. Utilization a proper delivery system for incorporation of drugs has a major impact on drug delivery and such a system should be formulated for prolonged drug retention in oral cavity. OBJECTIVES The aim of the...

OBJECTIVE We tested the hypothesis that carvedilol, a beta-adrenoceptor and alpha-adrenoceptor antagonist with potent antioxidant property, could inhibit tumor necrosis factor-alpha (TNF-alpha)-induced endothelial adhesiveness to human mononuclear cells (MNCs), an early sign of atherogenesis. METHODS AND RESULTS Circulating MNCs were isolated from the peripheral blood of healthy subjects. Com...

The regulation of hematopoietic stem cells (HSCs) depends on the integration of the multiple signals received from the bone marrow niche. We show the relevance of the protein tyrosine phosphatase PTPN13 and β-catenin as intracellular signaling molecules to control HSCs adhesiveness, cell cycling, and quiescence. Lethally irradiated mice transplanted with Lin(-) bone marrow cells in which PTPN13...

The bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F-actin filaments. The molecular composition and structure of the SZ were extensively investigated...

The purpose of this study was to compare the degree of erythrocyte adhesiveness/aggregation (EAA) reduction of two low-density lipoprotein (LDL) apheretic procedures, namely direct adsorption of lipoproteins (DALI) and dextran sulfate adsorption (DSA). A significant (P < 0.001) reduction of EAA was noted in six hypercholesterolemic patients who underwent a total of 40 apheretic sessions and no ...

Retinal adhesion deteriorates rapidly after enucleation. We have developed a new in vitro method for measuring retinal adhesiveness in the rabbit that is faster than previous models, and can monitor changes sooner after enucleation. We used the percentage of retina which retained pigment after peeling from the RPE as a quantitative measure of retinal adhesiveness. We found that the failure of a...

In the experiments reported below, the inner aspects of the pinnae of rabbits' ear~ were treated for 1 hour with the carcinogen 7,1~-dimethylbenzanthracene, 1 per ce~t ~n acetone. Other ears were treated with the nonearcinogen anthracene in aeetone, and still other ears were left untreated. Discs of skin were removed from these ears, and the relative adhesiveness of the epidermal cells was dete...

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