Although adipose tissue is an expandable and readily attainable source of proliferating, multipotent stem cells, its potential for use in regenerative medicine has not been extensively explored. Here we report that adult human and mouse adipose-derived stem cells can be reprogrammed to induced pluripotent stem (iPS) cells with substantially higher efficiencies than those reported for human and ...

Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. Although numerous immortalized adipogenic cell lines have been established, adipose-derived stem cells from the stromal vascular fraction of subcutaneous white adipose tissues provide a reliable cellular system ex vivo much closer to adipose dev...

Adipose tissue plays a key role in the development of insulin resistance and its pathological sequelae, such as type 2 diabetes and non-alcoholic fatty liver disease. Dysfunction in the adipose tissue response to storing excess fatty acids as triglyceride can lead to adipose tissue inflammation and spillover of fatty acids from this tissue and accumulation of fatty acids as lipid droplets in ec...

Background  Buccal fat pad is known as anatomic structure in between the masticatory muscles that separate these muscles from each other and surrounding bone. Ease of access, through the intraoral route makes it suitable choice for obtaining adipose derived stem cells. Methods  Intraoral incisions for getting access to the buccal fat of pad are; Retromolar incision, incision in medial of pt...

Patients with degenerative disc disease (DDD) experience serious clinical symptoms, including chronic low back pain. A series of therapies have been developed to treat DDD, including physical therapy and surgical treatment. However, the therapeutic effect of such treatments has remained insufficient. Recently, stem cell‑based therapy, in which stem cells are injected into the nucleus pulposus i...

Adipose-derived stem cells have been applied for the treatment of various diseases. This study was performed to fabricate the three-dimensional stem cell spheroids with adipose-derived stem cells and to evaluate the effects of cell number and culture duration on the viability and the osteogenic differentiation of three-dimensional cultures. Adipose-derived stem cells were purchased, and stem ce...

Dallas, Texas; and Lyon, France Summary: Adipose-derived stem cells are multipotent cells that can easily be extracted from adipose tissue, are capable of expansion in vitro, and have the capacity to differentiate into multiple cell lineages, which have the potential for use in regenerative medicine. However, several issues need to be studied to determine safe human use. For example, there are ...

Interdependency exists between adipogenesis, the formation of fat tissue from stem cells, and osteogenesis, the formation of bone from its stem-cell precursors. Adipose-derived adult mesenchymal stem cells may be useful for future bone regeneration and tissue engineering. This review discusses potential future relationships between autogenous adipose-derived stem cells, growth factors and resor...

Citation: Gojanovich AD, Gimenez MC, Masone D, Rodriguez TM, Dewey RA, Delgui LR, Bustos DM and Uhart M (2018) Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-Staining. Front. Cell Dev. Biol. 6:33. doi: 10.3389/fcell.2018.00033 Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-S...