INTRODUCTION: Anal traumas, such as blunt and penetrating injuries to the perineum, can disrupt anal sphincter cause considerable morbidity. Non-invasive trauma therapies have been widely developed, including growth factors administered platelet-rich plasma (PRP) stromal vascular fraction (SVFs) containing adipose[1]derived stem cells (ASCs) limited factor. This study aimed assess PRP SVF effec...

Major limitations in the effective treatment of neurological cancer include systemic cytotoxicity chemotherapy, inaccessibility, and inoperability. The capability to successfully target a drug tumor site(s) without incurring serious side effects—especially case aggressive tumors, such as glioblastoma neuroblastoma—would represent significant breakthrough therapy. Orthotopic systems, capable sto...

INTRODUCTION Adipose tissue transplantation is one of the standard procedures for soft-tissue augmentation, reconstruction, and rejuvenation. However, it is unknown as to how the graft survives after transplantation. We thus seek out to investigate the roles of different cellular components in the survival of graft. MATERIALS & METHODS The ratios of stromal vascular fraction (SVF) cellular co...

In tumors, cross talk between malignant and non-malignant cells (stroma) influences tumor growth, angiogenesis and metastasis. Stromal cells in tumors typically include vascular cells, fibroblasts and a heterogeneous population of inflammatory cells. Adipocytes may also be present. Adipose tissue is perhaps the least studied stromal cell "compartment" despite the fact that some tumors, particul...

Adipose-derived stem cells (ASCs) are multipotent mesenchymal stem cells obtained from stromal-vascular fraction of adipose tissue. ASCs are a promising resource for cell therapy due to their simple isolation, extensive expansion potential, and low immunogenicity. ASCs repair and regenerate damaged tissue by direct differentiation, whereas many other approaches rely on the secretion of paracrin...

We present a series of ten patients with non-reconstructable peripheral vascular disease (PVD), secondary to arteriosclerosis (AS) and/or diabetes mellitus (DM), treated with local injection of non-expanded autologous, adipose-derived stromal vascular fraction (SVF) cells for the purposes of enhancing neovascularization and chronic wound healing. Adipose tissue was surgically harvested and proc...

Engineering functional tissues of clinically relevant size (in mm-scale) in vitro is still a challenge tissue engineering due to low oxygen diffusion and lack vascularization. To address these limitations, perfusion bioreactor was used generate contractile engineered muscles 3 mm-thickness 8 mm-diameter. This study aimed upscale the process 50 mm diameter by combining murine skeletal myoblasts ...