This paper reports the evidence for the existence of multiple sites of action of aflatoxin B, in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B, injection (0.3 mg/lOO g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

Aflatoxin contamination of corn in the field is influenced by several factors. In the southern U.S., insect populations are usually large every year. Drought caused by warmer and drier than normal weather is conducive to A. flavus infection and aflatoxin contamination of corn, Zea mays L. When loose-husked hybrids are used in the southern U.S., they accentuate insect damage and aflatoxin contam...

This study was conducted to assess major Aspergillus species and aflatoxins associated with groundnut seeds and cake in Eastern Ethiopia and evaluate growers' management practices. A total of 160 groundnut seed samples from farmers' stores and 50 groundnut cake samples from cafe and restaurants were collected. Fungal isolation was done from groundnut seed samples. Aspergillus flavus was the dom...

This paper reports the evidence for the existence of multiple sites of action of aflatoxin B1 in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B1 injection (0.3 mg/100 g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

The present investigation is an attempt to evaluate the possible ameliorative effect of turmeric extracts/curcumin on aflatoxin induced hemolysis in vitro. Blood samples were collected from healthy adult human beings (25-30 years old) in EDTA vials and were used for preparation of RBC suspension in saline. Saline suspension of RBC was treated with aflatoxin (0.5-2.0 microg/mL) with and without ...

A preliminary approach to achieve compatible simultaneous extraction of aflatoxin and residual oil from pressed oil meals was conducted to determine the minimum amount of water to be added to ethanol versus necessary time to achieve complete removal of aflatoxin. Commercial anhydrous, 96, 93 and 90°GL ethanol were utilized in trials with Soxhlet extractors. Commercial anhydrous ethanol did not ...

Of 120 isolates of the Aspergillus flavus group from pecans used in bakery products, 85 were shown to produce aflatoxin on yeast extract sucrose medium. Extracts from moldy sections of raw pecans obtained commercially at the retail level showed aflatoxin-like spots on thin-layer chromatography. Cooked (autoclaved) pecans inoculated with toxigenic isolates of A. flavus were also good substrates ...

PksA catalyzes the formation of the polyketide backbone necessary for aflatoxin biosynthesis. Based on reporter assays and sequence comparisons of the nor1-pksA intergenic region in different aflatoxin-producing Aspergillus species, cis-acting elements for the aflatoxin pathway-specific regulatory protein, AflR, and the global-acting regulatory proteins BrlA and PacC are involved in pksA promot...

Soil moisture and soil temperature affect pre-harvest infection with Aspergillus flavus and production of aflatoxin. The objectives of our field research in Niger, West Africa, were to: (i) examine the effects of sowing date and irrigation treatments on pod yield, infection with A. flavus and aflatoxin concentration; and (ii) to quantify relations between infection, aflatoxin concentration and ...

BACKGROUND Aflatoxins cause health hazards to human and animals and has also economical problems. Therefore, the detoxification effect of citric acid was investigated in rice as the main food of Iranian people. METHODS Initially 275 samples of rice were examined for aflatoxins by HPLC. The aflatoxins contaminated samples were later treated by aqueous citric acid and detoxification of aflatoxi...