In man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. This report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. The gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. In liver, kid...

There has been much speculation that enzymes change the nature of the transition state for phosphoryl transfer from the dissociative transition state observed in solution reactions to an associative transition state at the enzyme's active site. This proposal can be tested by comparing linear free energy relationships (LFERs) for nonenzymatic and enzymatic reactions, provided that the specificit...

The effect of electrostatic interactions on the transition-state character for enzymatic phosphoryl transfer has been a subject of much debate. In this work, we investigate the transition state for alkaline phosphatase (AP) using linear free-energy relationships (LFERs). We determined k(cat)/K(M) for a series of aryl sulfate ester monoanions to obtain the Brønsted coefficient, beta(lg), and com...

background: halothane is the routine used inhalation anesthetic drug in the world but at present, there is an increasing interest for using intravenous anesthetics like propofol. the purpose of this study was to compare these anesthetic effects on hepatic enzymes.methods: from 2006 to 2007, this study was performed on fifty eight patients classified in class i of american society of anesthetics...

As phosphate derivatives form such important intermediates between the functioning of tissues and the metabolism which supports function, we decided to investigate the levels of certain phosphates in brain slices before and during metabolism in vitro. Investigation of this type appeared all the more necessary because there is now an extremely interesting group of observations on the levels of p...

Pirie, van Heyningen & Flanders (1955) showed that the concentrations of certain acid-soluble phosphates in the lens, when estimated by the enzymic method of Slater (1953), were much lower than the concentrations reported by Nordmann & Mandel (1952), who used the barium-fractionation method of LePage (1948, 1949). This method has, until recently, been the accepted and standard one for estimatio...

conclusions in critically ill patients, the symptoms of hypophosphatemia may not be apparent, but clinicians should be vigilant about this complication during therapy. in cases of severe symptoms (e.g., cardiopulmonary distress, anemia and thrombocytopenia, or rhabdomyolysis), phosphate therapy under close surveillance is warranted. introduction although asymptomatic hypophosphatemia is a commo...