The use-dilution test for evaluating the effectiveness of disinfectants against bacteria was modified to determine the effectiveness of disinfectants against a group of viruses. Modifications were kept to a minimum to retain the general principles of the test and thereby retain the test's familiarity among testing laboratory personnel. Modifications included the use of a standard allantoic flui...

Transfer of iron from native porcine uteroferrin to apotransferrin was investigated using EPR spectroscopy. Purple (oxidized) or pink (reduced) forms of uteroferrin were incubated with porcine or human apotransferrin under conditions of temperature (37 degrees C) and pH (6.8) approximating those found in the allantoic fluid of the pregnant sow. Studies were also performed in the presence of med...

Youngner, Julius S. (University of Pittsburgh, Pittsburgh, Pa.), and Marion E. Kelly. Inhibition by exogenous interferon of replication of poliovirus ribonucleic acid in chick brain. J. Bacteriol. 90:443-445. 1965.-The replication of poliovirus was studied after the intracerebral inoculation of infectious ribonucleic acid (RNA) into the brains of 2-day-old chicks; these animals are not suscepti...

Preparations of Type B influenza virus, propagated in the embryonated egg and obtained in the form of allantoic fluid, were found after heating at 56 degrees C. for 30 minutes to retain the capacity to agglutinate erythrocytes but no longer measured specific antibody when used as antigen in titrations of serum antibody. The dissociation of the two activities suggests the presence in such virus ...

Coronavirus defective RNAs (D-RNAs) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. D-RNA CD-61 derived from the avian coronavirus infectious bronchitis virus (IBV) was used as an RNA vector for the expression of chicken gamma interferon (chIFN-gamma). D-RNAs expressing chIFN-gamma were shown to be capable of rescue, replication, and...

The suggestion of Duesberg (1) that prolonged ingestion of allylisopropylacetylcarbamide (Sedormid)’ may have induced acute porphyria in a human led later workers to produce this disorder in rabbits (2) and rats (3) by oral administration of the drug. Although this experimental procedure has facilitated the study of acute porphyria, the possibility remains that some of the porphyrin excreted in...

Different physical factors like aeration, temperature and shaking culture conditions were applied to the culture and observation of growth was studied for 72 hours of incubation. Similarly the Mycoplasma mycoides subspecies capri was grown in PPLO medium enriched with different concentrations (5, 10 and 20%) of serum from horse, bovine and allantoic fluid, along with usual concentration of yeas...

T H E chemical composition of the foetal fluids in the rabbit was studied by Davies & Routh (1957). These investigators showed that the amniotic and exocoelomic fluids resembled extracellular fluid in their ionic composition whereas the allantoic fluid more closely resembled intracellular fluid. These findings were confirmed by Dickerson & McCance (1957). An experimental study of the trans-memb...

Abstract The differences between the microbial communities in reproductive tissues, and amniotic allantoic fluids of cattle have previously been investigated. Our objective was to provide insight into across tissues gilts during mid- late-gestation. Specifically, we characterized microbiomes vagina, cervix, endometrium, chorion, fluids. Duroc x Landrace Yorkshire (n = 12) free physical, health ...