Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to a wide range of antimicrobial agents including beta-lactams, aminoglycosides, macrolides, and polymyxins. We used Tn5-OT182 to mutagenize B. pseudomallei to identify the genes involved in aminoglycoside resistance. We report here on the identification of AmrAB-OprA, a multidrug efflux system in B. pseud...

Extreme antibiotic resistance in bacteria is associated with the expression of powerful inactivating enzymes and other functions encoded in accessory genomic elements. The contribution of core genome processes to high-level resistance in such bacteria has been unclear. In the work reported here, we evaluated the relative importance of core and accessory functions for high-level resistance to th...

INTRODUCTION Methicillin-resistant Staphylococcus aureus (MRSA) plays an important role in causing many serious nosocomial infections. In this study, the antimicrobial susceptibility and the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus was investigated from two university hospitals of Zanjan province of Iran. ...

conclusions it seems that besides the variety and prevalence of blatem and blactx, enormous mechanisms such as porin and leaking systems (efflux pumps) are responsible for the information of the a. baumannii resistance to disinfectants. the study on an accurate consideration of the resistance in strains and other microorganisms is advised. results in the current study, 131 a. baumannii isolates...

blast (magnaporthe grisea) is the most important disease of rice in mazandaran province, north of iran, which causes severe damages annually on susceptible cultivars. tarom mahalli is a local aromatic cultivar with high cooking quality and marketability, but very susceptible to blast. to protect this cultivar and similar susceptible cultivars against rice blast, farmers have to use high amounts...

this study aims at molecular identification of salmonella infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. a total of 46 salmonella-suspected samples isolated from backyard chickens of northern iran were collected. serotyping was done by the traditional method and then confirmed by pcr. antimicrobial susceptibility of the isolates against 13 antim...

the stripe (yellow) rust in wheat is one of the most important plant diseases in iran. since 1993 several epidemics have occurred in iran causing the breakdown of widely util-ized sources of resistance in wheat cultivars. twenty-seven pathotypes were identified during 2003 and 2004 in greenhouse tests. pathotypes 6e6a+, 6e22a+, 6e130a+, 6e134a+, 6e142a+, 6e158a+, 134e130a+ and 134e142a+ were mo...

Compost is widely used for gardening. Growers can choose to buy compost from markets or make at home. Potential exposure of users pathogens through composting includes ingesting foodborne and inhaling airborne pathogens. This study compared the abundances genetic markers five opportunistic in backyard commercial composts, as well an immature swine mortality compost. We found that ttrC Salmonell...

Aminoglycoside antibiotics have been shown to induce adaptive resistance in Pseudomonas aeruginosa in vitro and in a mouse model of infection, but adaptive resistance has not been described in human infections. Seven patients with cystic fibrosis were treated with inhaled tobramycin to determine whether adaptive resistance occurred in P. aeruginosa in their sputum. In three patients who had not...