We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly simplifies oligosaccharide purification on C-18 resin. Employing ceric ammonium nitrate as oxidative reagent to remove the p-NP group unexpectedly also removed the glucuronic...

L-arginine, an amino acid with a growing range of applications within the pharmaceutical, cosmetic, food, and agricultural industries, can be produced by microbial fermentation. Although it is the most nitrogen-rich amino acid, reports on the nitrogen supply for its fermentation are scarce. In this study, the nitrogen supply for the production of L-arginine by a genetically modified Escherichia...

The yeast Saccharomyces cerevisiae is a potential candidate for converting waste resources into food-grade single cell protein (SCP). Food fermentation broth rich in lactic acid was tested as an alternative substrate source S. this study. An electrodialysis (ED) system developed to recover lactate, acetate and ammonium ions (La-, Ac- NH4+) from the broth. First, we evaluated resilience under va...

Butyl lactate is a green solvent produced from renewable materials through the reaction of ammonium with n-butanol. It could be source material for valuable products such as propylene glycol, acrylic acid, its derivatives, and cyclic monomer polylactic acid (PLA)—lactate. In this study, we present novel non-catalytic interactions n-butanol carried out in temperature range 130–170 °C closed syst...

We examined how different media composition of rewarming solutions affected ammonium detoxification function, urea synthesis and the viability of hepatocytes after 72 hs of cold storage in UW solution. Freshly isolated rat hepatocytes were incubated at 37 C in a cell culture medium (MEM-E) with 3 mM glycine, 5 mM fructose and 2.5 mM adenosine (group 1) and in Krebs-Heinseleit buffer with 3 mM g...

1. The rate of removal from the circulation of an intravenous lactic acid load has been studied in conscious rats, previously subjected either to bilateral nephrectomy or to a sham operation. 2. In rats with normal blood pH, the apparent contribution of the kidneys to removal of the lactic acid load is 30%; less than 12% of the renal contribution is attributable to urinary excretion. 3. In bila...

In the light of the occurrence of L-lactate dehydrogenase inside the mitochondrial matrix, we looked at whether isolated rat liver mitochondria can take up and metabolize L-lactate, and provide oxaloacetate outside mitochondria, thus contributing to a partial reconstruction of gluconeogenesis in vitro. We found that: (1) L-lactate (10 mM), added to mitochondria in the presence of a cocktail of ...

Recently, the potential for biochar use to recapture excess nutrients from dairy wastewater has been a focus of a growing number of studies. It is suggested that biochar produced from locally available excess biomass can be important in reducing release of excess nutrient elements from agricultural runoff, improving soil productivity, and longterm carbon (C) sequestration. Here we present a rev...

It was believed for a long time that mRNA is very unstable, and can not be used for therapeutic purposes. In the last decade, however, many research groups proved its transfection feasibility along with advantages and applications. Our investigation is aimed at establishing a potent and efficient mRNA delivery system. We previously reported that an inorganic-organic hybrid carrier by exploiting...