It is concluded from experiments with (S)-[C015NH2]glutamine and [15N]ammonium sulphate that the biosynthesis of iodinin (1), in Brevibacterium iodinum, and of phenazine-1-carboxylic acid (7), in Pseudomonas aureofaciens, is primarily from a single nitrogen source (glutamine) and that the phenazines are constituted by combination of two units of the same precursor, as 5. Some observations are m...

Platelet releasing activity was found in sera fractionated by 30% ammonium sulphate in seven of 11 patients with systemic rheumatoid vasculitis, two of 16 patients with cutaneous rheumatoid vasculitis, and three of 18 patients with clinically uncomplicated rheumatoid arthritis. Serial studies in three patients suggest a key role of platelet activation by serum factors in the pathogenesis of sys...

The amino acid composition and preparation of crystals for X ray diffraction analysis of guanylonbonuclease isolated from Streptomyces aureofa­ ciens is described. Single crystals have been grown from ammonium sulphate solutions. The crystals are orthorombic, space group P 2i2]2, cell dimmensions a = 6.47 nm, b = 7.89 nm, c = 3.93 nm. There are two molecules of a molecular weight of 10 600 in t...

A cohaemolysin from the culture supernate of a canine pathogenic group G streptococcus (S. canis) was purified to electrophoretic homogeneity. The purification procedure involved ammonium sulphate precipitation, ultrafiltration, gel filtration and preparative isoelectric focusing. The cohaemolysin consisted of a single polypeptide chain, 18.6 kDa, with an isoelectric point at pH 5.1. The protei...

1. A method is described for partial purification of the total aldolase of rabbit brain by ammonium sulphate precipitation followed by fractionation of the aldolase into five electrophoretically distinct forms. 2. The method allows a laboratory-scale preparation of the five aldolases. 3. With the exception of band 3 the fructose 1,6-diphosphate/fructose 1-phosphate activity ratios of the isolat...

Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. For this purpose, ammonium sulphate was selected as a constant nitrogen source. The effect of different nitrogen sources was also investigated with glucose as a constant carbon source. The production of L-lysine was exam...

Removal and recovery of nitrogen with the Aqua2®N-process demonstrated The overall goal RE-Fertilize project was to demonstrate a new removal process for nitrogen, producing product suitable fertilizers, example. In project, demonstration plant has been built, in operational environment rate >95% ammonium nitrogen. produced sulphate evaluated its agronomic effect proven. A conceptual design ...

1. The activity of phosphofructokinase in sheep liver was found to be dependent on the composition and molarity of the buffer used in extraction. Under optimum conditions a value of 4-7mumoles/min./g. wet wt. of tissue was obtained. 2. The enzyme was purified 480-fold by a combination of ammonium sulphate fractionation, heat treatment in the presence of ethanol, DEAE-cellulose chromatography an...

AIMS To evaluate caffeine degradation and nitrogen requirements during Aspergillus tamarii growth in submerged culture. METHODS AND RESULTS Aspergillus tamarii spores produced on a coffee infusion agar medium added with sucrose were used. Several caffeine and ammonium sulphate concentrations (0-1 and 0-1.36 g l-1, respectively) were tested simultaneously on fungal biomass production and caffe...

In our previous work we purified and characterized the cathepsins from the small intestine of pig and rat 2. Colon is a part of digestive tract which morphologically and functionaly differs from the small intestine. For this reason we isolated and characterized the cathepsin from the mucosa of rat colon to see whether there are some differences in properties of both enzymes. Wistar albino rats,...