Fungi utilize polysaccharide substrates through extracellular digestion catalyzed by secreted enzymes. Thus far, protein secretion by the filamentous fungus Aspergillus niger has mainly been studied at the level of individual proteins and by genome and transcriptome analyses. To extend these studies, a complementary proteomics approach was applied with the aim to investigate the changes in secr...

Abstract. Anastasia L, Kodrat EA, Victor H, Sanjaya A, Pinontoan R. 2022. Isolation and characterization of indigenous amylolytic enzyme-producing Aspergillus sp. from sweet-flavored tapai. Biodiversitas 23: 5580-5586. are able to produce abundant enzymes that may have a crucial role in producing the sweet flavor This study was aimed evaluate activity isolated widely known tapai Jember, East Ja...

In germinating seeds under unfavorable environmental conditions, the mobilization of stores in the cotyledons is delayed, which may result in a different modulation of carbohydrates balance and a decrease in seedling vigor. Tall fescue (Festuca arundinacea Schreb.) caryopses grown at 4°C in the dark for an extended period in complete absence of nutrients, showed an unexpected ability to survive...

Screening of Bacillus subtilis strains capable producing extracellular hydrolases, including lipolytic enzyme was carried out. Within the scope this study, total 61 were isolated from various domestic specific environmental samples such as seawater, soil, Jeju ranch, hot spring, salt farm, and fermented food identified based on 16S rRNA sequences. Among isolates, 4 had lipase activity. The grow...

HORN. B. W. 1985, Association of Candida gllillier/llolldii with amylolytic filamentous fungi on preharvest corn, Can, J, MicrobioL 31: 19-23, Candida gllillier/nondii var. gllillier/llondii was the dominant yeast isolated from preharvest Georgia corn and comprised 3,6-47.0% of the total fungi as measured by dilution plating. The yeast was confined mainly to kernels visibly molded by filamentou...

Digestive enzymatic activity and nutritional responses of Autographa gamma (L.) (Lepidoptera: Noctuidae), an important insect pest of sugar beet, on nine sugar beet cultivars (Peritra, Karolina, Paolita, Lenzier, Tiller, Ardabili, Persia, Rozier, and Dorothea) were studied. The highest proteolytic activity of fourth and fifth instar of A. gamma was in larvae fed on cultivar Persia. The highest ...

Sweet potato (Ipomoea batatas L.) roots contain amylolytic enzymes, which hydrolyze starch thus having the potential to affect the viscosity of sweet potato porridges provided the appropriate working conditions for the enzymes are attained. In this study, the effect of sweet potato variety, postharvest handling conditions, freshly harvested and room/ambient stored roots (3 weeks), and slurry so...

Amylolytic abzymes are a new type of catalytically active human antibodies that have been found recently. Various immunoglobulins from the sera of patients with autoimmune diseases and human milk were found to possess amylolytic activity, which is expressed in their ability to hydrolyze ƒ¿-(1,4)D-glucosyl linkages of maltooligosaccharides, starch, glycogen, and several artificial substrates. Pu...

Thermoactinomyces vulgaris strain 5 produced two electrophoretically different alpha-amylases. Precipitation with ammonium sulfate and acetone did not alter the electrophoretic mobilities of either amylase isoenzyme. Patterns of the hydrolysis products of amylose by the two amylase isoenzymes were essentially identical.