Catalase activity of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine was studied. The high activity of the enzyme in cell-free extracts of both bacterial strains was determined (1745.21154.67 and 873.1172.23 U×mg protein for D. piger Vib-7 and Desulfomicrobium sp. Rod-9, respectively). The effect of different tempe...

Hepatic catalase activity in unanesthetized man has been de termined by measuring the disappearance rate of hydrogen perox ide spectrophotometrically in homogenates from needle biopsy specimens of liver. Ten healthy male prisoners, 59 patients with advanced cancer, and 60 cadavers shortly after death with cancer were studied. There is a wide range of catalase activity in normal human liver. Cat...

Previous work with Pseudomonas aeruginosa showed that catalase activity in biofilms was significantly reduced relative to that in planktonic cells. To better understand biofilm physiology, we examined possible explanations for the differential expression of catalase in cells cultured in these two different conditions. For maximal catalase activity, biofilm cells required significantly more iron...

Background and objectives: A key aspect of cisplatin-induced hepatotoxicity is oxidative stress. The current study was conducted to show, for the first time, the restoring and synergistic effects of morin and hesperidin against oxidative stress in hepatotoxicity. Methods: Fourty-two Wistar albino rats were randomly divided into seven groups: group A (control), ...

Catalases are widely used in many scientific areas. A catalase gene (Kat) from Geobacillus sp. CHB1 encoding a monofunctional catalase was cloned and recombinant expressed in Escherichia coli (E. coli), which was the first time to clone and express this type of catalase of genus Geobacillus strains as far as we know. This Kat gene was 1,467 bp in length and encoded a catalase with 488 amino aci...

objective(s):gentamicin sulphate (gs) nephrotoxicity seems to be related to the generation of reactive oxygen species. there is evidence that oxygen preconditioning increases the activity of antioxidant enzymes. materials and methods: forty eight female rats were divided into 6 groups (n=8) as follows: group 1 was the control, group 2 received daily gs, groups 3,4 and 5 received oxygen 2 hr/day...

The s.c. injection of talc, MgSiO3, into mice results in a de crease of hepatic catalase activity. A transient, but marked rise in catalase activity was observed on the 4th day following talc injection. This rise in activity was manifest primarily in the mitochondrial fraction. A concomitant increased incorporation of radioactive leucine occurred in the microsomal fraction. The relationship bet...

Oxidative stress and reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), which is detoxified by catalase, are implicated in fetal death and birth defects, but embryonic levels of catalase are only about 5% of adult activity, and its protective role is unknown. Our approach involved the use of mice genetically modified to either: (1) express low levels of endogenous catalase (acatala...