Mallory's triple staining is a histochemical technique used mainly for analysing connective tissues and glands and other tissues. We have described the differences in the nuclear staining between eutopic and ectopic endometrium as well as endometrial hyperplasia and adenocarcinoma using the Mallory's method. The ultrastructural differences between eutopic and ectopic endometrium have been detec...

Clinical mycology laboratories now prepare fewer tease mounts of filamentous fungi but increasingly use a variety of tape touch or Scotch Tape methods to microscopically study molds. Mounts are often prepared with clear cellophane tape by a procedure similar to that described in Larone’s Third Edition of Medically Important Fungi: a Guide to Identification (1). Because it is not the most common...

The present study focuses on predicting the concentration of intracellular storage polymers in enhanced biological phosphorus removal (EBPR) systems. For that purpose, quantitative image analysis techniques were developed for determining the intracellular concentrations of PHA (PHB and PHV) with Nile blue and glycogen with aniline blue staining. Partial least squares (PLS) were used to predict ...

In Rhododendron spp. and Ledum groenlandicum a callose wall is laid down around the zygote in the first 2 days after fertilization. The periodic acid/Schiff-positive, aniline blue-fluorescence-positive callosic wall is initiated adjacent to the degenerating synergid, extends to cover the entire zygote surface, and remains visible during the initiation of embryogeny as the zygote elongates befor...

We explain the development of an ultra-sensitive molecularly imprinted polymer-based electrochemical sensor for rapid and selective determination bisphenol A (BPA) in human serum water samples. Electropolymerization functional monomer aniline was performed presence BPA by cyclic voltammetry (CV) to prepare a poly(aniline) based GCE (MIP(ANI)/GCE). The developed MIP surface characterized using F...

Accurate prognosis of male fertility based on semen measurements is still not straightforward. This study was designed to identify the best predictors of fertility and to develop a multiple regression model predicting fertility using selected parameters of semen analysis. The predictive value of standard semen parameters and selected functional tests were studied in 113 fertile men and in 109 s...

Ethical disclosures The genus Rhizoctonia DC (1805) has long been studied as an important soilborne pathogen that causes a wide variety of symptoms because it is a non-specialized pathogen3. Rhizoctonia sensu lato is characterized by the lack of conidiogenous cells and this taxon is composed of two groups based on the number of nuclei per cell: the multinucleate group that belongs to Rhizoctoni...

OBJECTIVES To evaluate the incidence of functional and chromatin alterations on spermatozoids in patients grouped according to normality of standard semen parameters based on the 5th edition of the World Health Organization (WHO) guidelines. To identify and correlate the most frequently altered characteristics in the normal standard semen parameters sub-population. MATERIALS AND METHODS A pro...

The aniline–neon van der Waals complex has been investigated from a theoretical point of view. The intermolecular distance, structure and rotational constants in the ground electronic state have been obtained by ab initio calculations using second-order Møller–Plesset (MP2) theory. The potential energy surface has also been determined. It has been found that two conformers exist: the anti, wher...