The advent of metagenomics has greatly facilitated the discovery of enzymes with useful biochemical characteristics for industrial and biomedical applications, from environmental niches. In this study, we used sequence-based metagenomics to identify two antibiotic resistance enzymes from the secluded, lower convective layer of Atlantis II Deep Red Sea brine pool (68°C, ~2200 m depth and 250‰ sa...

Background: Florfenicol (Ff) is a synthetic antibiotic with a broad antibacterial spectrum and high therapeutic effectiveness that was specifically developed for veterinary use as well as, doxcycline is an antibiotic synthetically derived from oxytetracycline. Methods: In the present study, the in-vitro efficacy of Ff and doxcycline against Salmonella entertidis and Escherichia coli (Serotype O...

Wastewater reclamation for municipal irrigation is an increasingly attractive option for extending water supplies. However, public health concerns include the potential for development of antibiotic resistance (AR) in environmental bacteria after exposure to residual pharmaceuticals in reclaimed water. Though scientific studies have reported high levels of AR in soils irrigated with wastewater,...

the effect of fungicides on silkworm larvae was analyzed with regard to the fact that these beneficial insects are usually affected by fungicides, daily feeding on 1 and 2g/liter of carbendazim, a systemic fungicide, did not have significant effects on larval and pupal mortality. however the weight of the treated larvae showed considerable weight decrease up to 37% weight.. all the economic tra...

AIM To delay surgery until the patient is in a better condition, and thus to decrease postoperative morbidity. METHODS Using this algorithm we treated three patients aged 55, 75 and 80 years. In all three patients the clinical presentation was fever without a clear source of infection; all had nonspecific symptoms such as general malaise, dyspnea, and abdominal discomfort in the previous 15 d...

The accelerated growth of finfish aquaculture has resulted in a series of developments detrimental to the environment and human health. The latter is illustrated by the widespread and unrestricted use of prophylactic antibiotics in this industry, especially in developing countries, to forestall bacterial infections resulting from sanitary shortcomings in fish rearing. The use of a wide variety ...

The propagation of recombinant plasmids in bacterial hosts, particularly in Escherichia coli, is essential for the amplification and manipulation of cloned DNA and the production of recombinant proteins. The isolation of bacterial transformants and subsequent stable plasmid maintenance have traditionally been accomplished using plasmid-borne selectable marker genes. Here we describe a novel sys...

Parapneumonic empyema (PPE) is an important complication of pediatric pneumonia and leads to prolonged hospitalization and substantial short-term morbidity. Over the past decade, there has been vigorous debate into the optimal management of such patients leading to marked variation in care. Most of the debate has focused on the optimal drainage procedure, focusing on clinical trials that have a...