نتایج جستجو برای: antibody detection
تعداد نتایج: 718126 فیلتر نتایج به سال:
Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antib...
the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...
Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to gi...
In the biomedical field, end-point detection bioassays such as enzyme-linked immunosorbent assays (ELISAs) are essential tools because of their simplicity, high-throughput, and suitability for their use at the point-of-care. End-point bioassays are significantly constrained by the need of sample labeling with fluorescent or colorimetric tags for subsequent detection. A promising strategy to ove...
BACKGROUND Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. METHODS An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a bios...
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