OBJECTIVE To determine whether measurement of different markers of endothelial damage, activation of coagulation, and platelet activation might differentiate between patients with primary Raynaud's phenomenon (PRP), limited cutaneous and diffuse systemic sclerosis (lcSSc and dSSc), and healthy control subjects. METHODS Under carefully controlled conditions, fasting blood was drawn from 19 hea...

Genes encoding the four principal polypeptide domains (N, A1-B1, A2-B2, and A3-B3) of carcinoembryonic antigen (CEA) were synthesized and expressed in Escherichia coli as fusion products with bacterial CMP-KDO synthetase (CKS). The four synthetic fusion proteins were purified in high yield and used as targets in Western blots for 11 anti-CEA MAbs and to compete with immobilized CEA for binding ...

To determine if amplification of the C-erb-B2 proto-oncogene could be correlated with prognosis in carcinoid tumours, 49 pulmonary carcinoid tumours (26 typical, 23 atypical) were examined using a polyclonal antibody to the C-terminal peptide of the C-erb-B2 protein sequence. No C-erb-B2 gene product could be shown: the demonstration of C-erb-B2 does not seem to help, therefore, in determining ...

We are going to discuss in brief and without proof the equality case in Theorem 1.1. Before we can do this, we need to establish a notation: The notation (a1; a2; :::; an) (b1; b2; :::; bn) is going to mean that for every two numbers i and j from the set f1; 2; :::; ng ; we have aibj = biaj: Note that if all numbers b1; b2; :::; bn are nonzero, then (a1; a2; :::; an) (b1; b2; :::; bn) is equiva...

The cytochrome domain of flavocytochrome b2 (L-lactate dehydrogenase) was expressed in the bacterium Escherichia coli and a purification procedure was developed. When expressed in E. coli, the b2-cytochrome domain contains protohaem IX and has an electronic absorption spectrum identical with that of the cytochrome b2 'core' produced by proteolytic cleavage of the enzyme isolated from yeast. The...

Recent studies have shown that ephrin-B2 on sensory afferent fibers from the dorsal root ganglia (DRG) controls transmission of pain sensation to the spinal cord. We examined ephrin-B2 expression in mouse DRG and spinal cord using an ephrin-B2/ß-galactosidase chimeric allele. We found that ephrin-B2 is expressed exclusively in proprioceptive neurons and fibers in neonates, while expression in l...

Let X be an n–element finite set, 0 < k < n/2 an integer. Suppose that {A1, B1} and {A2, B2} are pairs of disjoint k-element subsets of X (that is, |A1| = |B1| = |A2| = |B2| = k,A1 ∩ B1 = ∅, A2 ∩ B2 = ∅). Define the distance of these pairs by d({A1, B1}, {A2, B2}) = min{|A1−A2|+ |B1−B2|, |A1−B2|+ |B1−A2|}. It is known ([2]) that this is really a distance on the space of such pairs and that the ...

OBJECTIVE Vascular expression of the B1 kinin receptor is markedly upregulated with left ventricular dysfunction and angiotensin-converting enzyme (ACE) inhibition, but its function remains unclear. Inhibitors of ACE potentiate bradykinin-mediated B2 receptor-dependent vasodilatation and tissue plasminogen activator (tissue-type plasminogen activator [t-PA]) release. We investigated the contrib...

A subset of integrin a subunits contain an I domain, which is important for ligand binding. We have deleted the I domain from the b2 integrin lymphocyte function-asssociated antigen-1 (LFA-1) and expressed the resulting non–I domain-containing integrin (DI-LFA-1) in an LFA-1-deficient T cell line. DI-LFA-1 showed no recognition of LFA-1 ligands, confirming the essential role of the I domain in ...

We show that CC chemokines induced a sustained increase in monocyte adhesion to intercellular adhesion molecule-1 that was mediated by Mac-1 (aMb2) but not lymphocyte function–associated antigen-1 (LFA-1; aLb2). In contrast, staining for an activation epitope revealed a rapid and transient up-regulation of LFA-1 activity by monocyte chemotactic protein-1 (MCP-1) in monocytes and Jurkat CCR2 che...