Metabolic inhibitors were added to a culture medium inoculated with theramlly stressed Vibrio parahaemolyticus to obtain information pertaining to biosynthetic processes required for recovery from heat damage. Ribonucleic acid and protein syntheses, in addition to membrane repair, were required during recovery of injured cells. Neither nucleic acid nor Mg2+ leakage was noted to occur during the...

The roles of anthranilate and histidine in the regulation of tryptophan synthesis were implicated by observations of antagonistic relationships with 5-methyltryptophan in Bacillus subtilis.

The question of the significance of free amino acids in soil or other cultural media has received attention in recent years in the fields of plant physiology (1, 3, 7, 13, 14, 15, 16, 17), soil microbiology (4, 5, 11, 16) and soil chemistry (9, 10,12). Although it originally appeared that free amino acids were not to be found in soils, later reports (2, 10) indicated they could be extracted wit...

BACKGROUND The incidence of invasive cancer within a mucinous cystic neoplasm of the pancreas varies between 6 and 36%. Polycystic ovarian syndrome is a disorder characterized by hyperandrogenism and anovulatory infertility. One surgical treatment that can restore endocrine balance and ovulation in polycystic ovarian syndrome is partial ovarian destruction. Successful pregnancies following prec...

By ROBERT SCHWARTZ AND WILLIAM DAMESHEK P RIOR TO 1949, the treatment of autoimmune hemolytic anemia ( AIHA) was frequently disappointing. Despite splenectomy and the use of blood transfusions, many patients had an unremitting and progressively deteriorating course which ended fatally. With the introduction of ACTH and cortisone, the treatment of this disorder was greatly facilitated and benefi...

We explored whether the combination of lurbinectedin (PM01183) with the antimetabolite gemcitabine could result in a synergistic antitumor effect in pancreatic ductal adenocarcinoma (PDA) mouse models. We also studied the contribution of lurbinectedin to this synergism. This drug presents a dual pharmacological effect that contributes to its in vivo antitumor activity: (i) specific binding to D...

BACKGROUND The antimetabolite 6-thioguanine (6-TG) has been used to treat both human and canine lymphoid malignancies. 6-TG has been shown to be epigenetically active as a demethylating agent in a human lymphoma cell line, causing downregulation of DNA methyltransferase 1 (DNMT1) through ubiquitin-targeted degradation. Zebularine (Zeb), a similar cytidine analog, also has demethylating activity...

Correspondence: Joyce O’Shaughnessy, M.D., Baylor-Sammons Cancer Center, 3535 Worth St., Collins 5, Dallas, Texas 75246, USA. Telephone: 214-370-1795; Fax: 214-370-1850; e-mail: joyce.o’[email protected] Received October 3, 2005; accepted for publication October 7, 2005. ©AlphaMed Press 1083-7159/2005/$12.00/0 Abstract Metastatic breast cancer (MBC) remains essentially incurable, and g...

BACKGROUND Maintaining homeostasis is the biological function of negative feedback, a process that plays a well-understood role in the biochemistry of antimetabolite drugs. An equally important property of living systems--the ability to respond to external stimuli by switching rapidly from one state to another--is mediated by positive feedback. Kinetic analysis of multi-enzyme biochemical pathw...