The Analytab system of 20 biochemical tests for identification of Enterobacteriaceae was evaluated in parallel with conventional tests on 128 Enterobacteriaceae, 5 Aeromonas, and 1 Yersinia enterocolitica. The results of tests for H(2)S and indole production, citrate utilization, lysine and ornithine decarboxylase, arginine dihydrolase, nitrate reduction, beta-galactosidase, and fermentation of...

inoculation of phosphate-solubilizing microorganisms along with rock phosphate is known to enhance the available p from soil. the success of such solubilization is largely dependent on the ability of the inoculant strain to survive under adverse environmental conditions. in this context, liquid inoculants are gaining importance and are becoming popular with longer shelf-life. in the present inv...

A detailed radiorespirometric and enzymological analysis was made of the wild-type and GFI strains of Thiobacillus A2 grown on numerous sugars. The wild-type used the EmbdenMeyerhof, Entner-Doudoroff and pentose phosphate pathways for glucose oxidation only after culture on glucose. The fast-growing strain GFI used all three pathways after growth on glucose, maltose or fructose. The wild-type g...

Cell walls from flesh, parenchyma cells, stone cells and skin were isolated from ripe and overripe Pyrus communis L. cv "De Cloche" using the phenol-buffer method. Pear polysaccharides were solubilized from cell walls by sequential extractions with aqueous solutions of ammonium oxalate, Na2CO3, and increasing concentration of NaOH, to explore overripening impact. Cell walls were also differenti...

It was investigated whether Escherichia coli cultured in a glucose-limited chemostat is able to grow with a series of sugars whose utilization is normally repressed during batch growth with glucose. Cells growing at dilution rates of 0.2, 0.3 and 0.6h-1 were able to immediately utilize and grow with fructose, mannose, maltose and ribose. Galactose was transported instantaneously but growth star...

Aldoses and alduronic acids can be simultaneously identified and quantitated by wide-bore capillary gas-liquid chromatography. The aldoses are converted to alditol acetates and the alduronic acids are converted to their corresponding N-propylaldonamide acetates. Standard mixtures containing as little as 100 ng each of arabinose, xylose, mannose, galactose, glucose, inositol, mannuronic, glucuro...

A reasonable degree of synchrony in the sporulation of Clostridium thermosaccharolyticum 3814 was obtained by using three 10% transfers of 8-hr cultures in a medium containing 0.5% L-arabinose, 0.5% peptone, 0.5% yeast extract, and Gc minerals. Sporulation was stimulated by L-arabinose and L-xylose, but was repressed by glucose, mannose, fructose, and D-pentoses. Sporulating cells were long and...

In the preceding paper (Siegel and Clifton, 1950) on the oxidative assimilation of carbohydrates by Escherichia coli considerations of growth, assimilation, and energetics made it evident that free energy relationships alone do not provide conclusive criteria concerning the extent of the assimilability of a carbohydrate utilized by the cell. This has been attributed to the more important role o...

A dynamic metabolic model is presented for Pediococcus pentosaceus producing lactic acid from lignocellulose-derived mixed sugars including glucose, mannose, galactose, arabinose, and xylose. Depending on the pairs of mixed sugars, P. pentosaceus exhibits diverse (i.e., sequential, simultaneous or mixed) consumption patterns. This regulatory behavior of P. pentosaceus is portrayed using the hyb...