The construction and screening of metagenomic expression libraries has great potential to identify novel genes and their functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotatio...

Currently, analysts receive enormous amount of information from multiple sensors and resources. In addition to knowledge bases and traditional databases, enormous volumes of unstructured information are now available and are presented to the analyst [1], [35], [26]. This presents a significant challenge since different pieces of information from several heterogeneous data sources must be fused ...

Understanding fate of antibiotic resistant bacteria (ARB) vs. their antibiotic resistance genes (ARGs) during wastewater sludge treatment is critical in order to reduce the spread of antibiotic resistance through process optimization. Here, we spiked high concentrations of tetracycline-resistant bacteria, isolated from mesophilic (Iso M1-1-a Pseudomonas sp.) and thermophilic (Iso T10-a Bacillus...

The increasing abundance of antibiotic resistance genes (ARGs), which are regarded as new pollutants, has raised public health concern. use antibiotics in aquaculture promoted the evolution and spread ARGs. occurrence ARGs attracted extensive attention. However, distribution transmission require further study. This study analyzed water sediment from intensive culture hybrid snakehead fish farm ...

Abstract An on‐farm solid–liquid separator (SLS) and rotary drum composter (RD) manure treatment system was monitored for its impact on antibiotic residues resistance genes (ARGs). Administered antibiotics were tracked, mass flows quantified. Total amounts of ARGs calculated from measured concentrations flows. Only oxytetracycline (OTC) sulfadimethoxine (SDM) detected in the influent. No β‐lact...

Antibiotic resistance genes (ARGs) may be introduced to agricultural soil through the land application of cattle manure. During a rainfall event, manure-borne ARGs infiltrate into subsurface and leach groundwater. The objective this study was characterize model vertical transport following beef manure on surface. In study, column experiments were conducted evaluate influence four ARGs: erm(C), ...

Androgen receptor (AR) signaling is a key pathway modulating prostate cancer (PCa) progression. Several steps in this pathway have been investigated in order to propose novel treatment strategies for advanced PCa. Total osteopontin (OPN) has been described as a biomarker for PCa, in addition to its role in activating the progression of this tumor. Based on the known effects of the OPNc splice v...

BACKGROUND Alcohol abuse and dependence are a serious public health problem. A large number of alcohol-regulated genes, (ARGs) are known to be influenced by alcohol use and withdrawal (AW), and recent evidence suggests that neuroadaptation to alcohol may be due in part to epigenetic changes in the expression of ARGs. Gabra4, which encodes the α4 subunit of GABAA receptors (GABAARs), is one of a...

Large-scale association studies are being undertaken with the hope of uncovering the genetic determinants of complex disease. We describe a computationally efficient method for inferring genealogies from population genotype data and show how these genealogies can be used to fine map disease loci and interpret association signals. These genealogies take the form of the ancestral recombination gr...

The use of augmented reality games (ARGs) in education has gained increased attention from curriculum developers, teachers, and researchers the past decade. Research findings show that ARGs can promote meaningful learning environments foster key competences for 21st century. This paper presents design process “EcoAegean”, an ARG mobile devices, which was implemented primary classroom to support...