Myosin V (myoV) is a two-headed myosin capable of taking many successive steps along actin per diffusional encounter, enabling it to transport vesicular and ribonucleoprotein cargos in the dense and complex environment within cells. To better understand how myoV navigates along actin, we used polarized total internal reflection fluorescence microscopy to examine angular changes of bifunctional ...

Physical interactions between distinct chromosomal genomic loci are important for genomic functions including recombination and gene expression, but the mechanisms by which these interactions occur remain obscure. Using telomeric association as a model system, we analyzed here the in vivo organization of chromosome ends of haploid yeast cells during interphase. We separately labeled most of the...

To determine the replicative mechanism for human cytomegalovirus (HCMV) DNA, field inversion gel electrophoresis was used to separate HCMV replicative DNAs during lytic infection. Unit-length circular HCMV genomes lacking terminal restriction fragments were detected starting 4 h after infection even when cells were treated with aphidicolin, phosphonoacetic acid, or cycloheximide. Viral DNA synt...

We have produced a high-resolution physical recombination map for tomato chromosomes by determining the frequency and distribution of recombination nodules (RNs) on tomato synaptonemal complexes (SCs). We present evidence that there is a 1:1 relationship between RNs and chiasmata. Every SC has at least one RN. There are no RNs at the ends of SCs, in kinetochores, or in the heterochromatic short...

BACKGROUND Biomechanics studies can help improve athletic performance. However, the biomechanics of knee joint ligament length changes and moment arms over six degrees of freedom (DOF) have yet to be established. OBJECTIVE To construct a knee model to investigate the length and moment arm changes of the anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), medial collateral lig...

Barrett's esophagus is a useful model for the study of carcinogenesis, as the metaplastic columnar epithelium that replaces squamous esophageal epithelium is at elevated risk for development of adenocarcinoma. We examined telomere length and chromosomal instability (CIN) in Barrett's esophagus biopsies using fluorescence in situ hybridization. To study CIN, we selected centromere and locus-spec...

The Index of Coordination has been currently used as a tool that measures the inter-arm lag time between propulsive phases in front crawl swimming. The aim of the present study was to assess the inter-arm coordinative structure during a 200m front crawl maximal effort, as well as to understand its interplay with the stroking parameters. One complete arm stroke cycle, without breathing, was digi...

Although there have been no significant studies of the effects of different coupling chemistries, spacer arm length, or the nature of the functional group on the performance of ultrafiltration membranes, there is an extensive literature on these parameters in the field of chromatography. An optimal spacer arm ensures that the functional groups are placed a suitable distance from the surface of ...

background: nonspecific symptoms are common in all areas of medicine. patients and caregivers can be frustrated when an illness cannot be reduced to a discrete pathophysiological process that corresponds with the symptoms. we therefore asked the following questions: 1) which demographic factors and psychological comorbidities are associated with change from an initial diagnosis of nonspecific a...