Pestivirus nonstructural protein 3 (NS3) is a multifunctional with protease and helicase activities that are essential for virus replication. In this study, we used combination of biochemical genetic approaches to investigate the relationship between positively charged patch on module NS3 function. The surface composed four basic residues, R50, K74 K94 in domain H24 structurally integrated cofa...

Seed-type vacuolar processing enzyme (VPE) activity is predicted to be essential for post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds. To test this hypothesis, we examined the protein profiles of developing and germinating seeds from Arabidopsis plants containing transposon-insertional knockout mutations in the genes that encode the two ...

By using degenerate primers and Inverse-PCR, two extracellular aspartic protease encodinggenes were identified and sequenced from two yeast species of oenological origin:Metschnikowia pulcherrima IWBT Y1123 named MpAPr1 and Candida apicola IWBT Y1384named CaAPr1. MpAPr1 is 1137 bp long and the mature protein consists of 362 amino acidswith a molecular weight of 39.2 kDa. The pre...