We investigate the interaction of atrial natriuretic peptide (ANP) brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) with their receptors (NPRA, NPRB and NPRC), as well as the proportion and localization of those receptors in the rat ciliary body. Binding assays and affinity cross-linking experiments demonstrated the presence of the NPRC receptor type. However, the three natr...

BACKGROUND The onset of symptoms is a critical point in the natural history of aortic stenosis and the cardinal indication for valve replacement. This study assessed the associations between natriuretic peptide levels, disease severity, and cardiac symptoms in aortic stenosis. METHODS AND RESULTS Seventy-four patients with isolated aortic stenosis underwent independent assessment of symptoms,...

Depressor and renal activities of atrial natriuretic factor-(99-126) were determined in conscious, unrestrained spontaneously hypertensive rats treated with a neutral endopeptidase inhibitor, SQ 29,072 (7-[[2-(mercaptomethyl)-1-oxo-3-phenylpropyl]amino]heptanoic acid). SQ 29,072 (100 mumol/kg i.v.) prolonged the transient depressor effects of the peptide for as long as 2 hours. During the first...

OBJECTIVES Our aim was to design, synthesize and test in vivo and in vitro a new chimeric peptide that would combine the beneficial properties of 2 distinct natriuretic peptides with a biological profile that goes beyond native peptides. BACKGROUND Studies have established the beneficial vascular and antiproliferative properties of C-type natriuretic peptide (CNP). While lacking renal actions...

Running title: Vascular interactions between ANP and NO Classification: Biological Sciences, Physiology Manuscript information: 21 pages, 6 figures; abstract with 245 words, 5151 words in total. Abbreviations footnote: ANP, atrial natriuretic peptide; CNP, C-type natriuretic peptide; NO, nitric oxide; GC-A, guanylyl cyclase-A; sGC, soluble guanylyl cyclase; PKG I, cGMPdependent Protein Kinase I...

Effects of intracerebroventricular (i.c.v.) treatment with atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) on the pressor response to i.c.v.-injected clonidine were investigated in conscious rats. I.c.v.-pretreatment with ANP (10 micrograms) or BNP (10 micrograms) inhibited the pressor response to i.c.v.-injected clonidine (10 micrograms). Systemic (i.v.) treatment with ANP...

BACKGROUND Brain natriuretic peptide (BNP) levels are used in the evaluation of patients with heart disease, yet there is little understanding of the effect of hypoxia on natriuretic peptide secretion. Furthermore, recent data suggest that oxytocin may mediate stretch-induced atrial natriuretic peptide (ANP) secretion. METHODS AND RESULTS Ten patients with cyanotic congenital heart defects an...