The Saccharomyces cerevisiae spore wall is a multilaminar coat that surrounds individual spores and protects them from environmental insult. Scanning electron microscopy reveals that the four spores of an ascus are connected by interspore bridges. Transmission electron microscopy of spores indicates that these bridges are continuous with the outer layers of the spore wall. In chs3 mutants, whic...

In the present study, the aim has been to investigate the interobserver reproducibility of DNA-image-cytometry (DNA-ICM) applied to routine Pap smears classified as Atypical Squamous Cells of Undetermined Significance (ASCUS) or higher lesions (ASCUS+). 202 Pap smears diagnosed as ASCUS or higher were included in the study. After cytological assessment, smears underwent restaining according to ...

Catinella olivacea is a discomycetous fungus often found fruiting within cavities in rotting logs. Because this habitat would lack the air currents upon which discomycete species normally rely for the dispersal of their forcibly ejected ascospores, we suspected an alternative disseminative strategy might be employed by this species. An examination of the development of the discomycetous ascomat...

www.sciencemag.org SCIENCE VOL 332 20 MAY 2011 885 COVER Phase contrast photomicrograph of a Schizosaccharomyces octosporus ascus, a sac-like cell that typically contains eight spores (each ~2 micrometers across). S. octosporus and other fi ssion yeasts are important models of eukaryote biology and have evolved a single-celled lifestyle independently from their budding yeast cousins. On page 93...

Mutants at a newly identified locus, DIS1 (disjunction), were detected by screening for mutants that generate aneuploid spores (chromosome VIII disomes) at an increased frequency. Strains carrying the partially dominant alleles, DIS1-1 or DIS1-2, generate disomes at rates up to 100 times the background level. Mitotic nondisjunction is also increased 10- to 50-fold over background. Half-tetrad a...

Human papillomavirus (HPV) has more than 100 different types, some of which are associated with cancer. The most common example is that cervical cancer, HPV16 and HPV18. Here, we performed next-generation sequencing (NGS) to type 2436 samples obtained from Korean women elucidate the correlation between multiple infections, virus cytology. NGS revealed types 58, 56, 16 were in high-risk (HR) whe...

The aim of this study was to compare the diagnostic efficacy colposcopic-directed biopsy and four-quadrant in detecting high-grade cervical intra-epithelial neoplasia (CIN). Women attending three women’s clinics for routine screening were recruited. Colposcopy arranged women with any cytologic abnormalities greater than atypical squamous cells undetermined significance (ASCUS), two consecutive ...

BACKGROUND The Norwegian Cervical Cancer Screening Program recommends screening every 3 years for women between 25 and 69 years of age. There is a large difference in the percentage of unsatisfactory samples between laboratories that use different brands of liquid-based cytology. We wished to examine if inadequate ThinPrep samples could be satisfactory by processing them with the SurePath proto...

Equations expressing the theoretical frequencies of twelve ascus-types in the tetrad analysis of a triply heterozygous diploid are described. Using these equations, a mapping procedure for a gene X, is proposed. The procedure requires that two genes, X and Y, of the same phenotype be heterozygous and that the map position of Y be known, and that another standard gene, Z, show an independent phe...