We recently isolated PsAD1 cDNA from pea (Pisum sativum L. cv. Alaska) seedlings, whose mRNA abundantly accumulated in dormant axillary buds and disappeared after decapitation [Madoka and Mori (2000) Plant Cell Physiol. 41: 274]. To further elucidate the function of PsAD1, we investigated the temporal and spatial distribution patterns of PsAD1 protein using Western blot and immunocytochemical a...

the present investigation was conducted to study the effects of type and concentration of auxin and cytokinin on callus induction of three strawberry (kurdistan, parose and camarosa) cultivars. for this purpose, leaf blade, nodal, petiole, receptacle, stamen and flower bud explants were cultured on murashige and skoog (ms) medium at three separate experiments included 2, 4 dichlorophenoxyacetic...

Dorsal and ventral pancreatic bud development from the endoderm requires inductive interactions with diverse mesodermal cell types and the action of transcription factors expressed within the endoderm. Presently it is unclear which mesodermal interactions activate which pancreatic transcription factors, and whether such inductions are common for initiating dorsal and ventral pancreas developmen...

Stem and root tumors and teratomata may be induced in tobacco plants (Nicotiana tabacum L., var. One Sucker) by total debudding. Intact plants or plants only decapitated produced no tumors or teratomata throughout their life time. These findings suggest a possibly important oncological relationship between normal growth and tumorous growth. Prevention of normal regeneration (apical and axillary...

Axillary bud growth – and specifically its release from apical dominance – has been a longstanding and complex area of research. Many phytohormone systems, as well as sugar signaling, have been implicated. Now, Holalu and Finlayson (see pages 943–952) have found compelling evidence that a much less-studied hormone in this context – abscisic acid (ABA) – may, in fact, be one of the first upstrea...

This study was carried out in order to determine what factors control the differentiation of certain neural crest cells in the chick embryo. Emphasis was placed on the morphologically and biochemically divergent sensory and sympathetic pathways of differentiation. Embryos were precisely stage according to Hamburger & Hamilton (1951) and it was observed that sensory ganglia with somites, explant...

During vertebrate embryogenesis, the muscle-specific helix-loop-helix protein myogenin is expressed in muscle cell precursors in the developing somite myotome and limb bud before muscle fiber formation and is further upregulated during myogenesis. We show that cis-acting DNA sequences within the 5' flanking region of the mouse myogenin gene are sufficient to direct appropriate temporal, spatial...

The endangered medicinal plant Eryngium maritimum L. faces significant natural and anthropogenic threats. Therefore, in vitro propagation is recommended for both conservation commercial purposes. aim of the study was to develop a series protocols seed disinfection, multiplication rooting, ex field adaptation. For explant length temperature three consecutive disinfectants were investigated. Macr...

Development of a simple and inexpensive method of suppressing axillary branching would be a matter of practical importance to tobacco growers. This has already been pointed out in a preliminary report of this work (10). Suckering or the removal of branches, is commonly practiced after topping, or decapitation of the tobacco plant at the bud or flower stage. More or less frequent suckering of th...