The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, orf12 (virB12) and orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether orf12 (virB12) encodes an essential part of the T4SS apparatus. The virB12 gene was found t...

21 One of the capabilities developed by bacteria is the ability to gain large fragments of DNA 22 from other bacteria or lose portions of their own genome. Among these exchangeable 23 fragments are the genomic islands (GIs). Nine GIs have been identified (GI) in Brucella, 24 being the genomic island 3 (GI-3) shared by two pathogenic species: B. melitensis and B. 25 abortus. GI-3 encodes mostly ...

WORTHINGTON, R. W., MULDERS, MARIA S. G., McFARLANE I. S. and BECKER DAPHNE. Serological titres following vaccination of sheep and goats with B. :nelitensis Rev 1 vaccine: Onderstepoort f. vet. Res. 40(1), 1-6 (1973) Rev 1 vaccination of sheep induced complement fixation titres to both Brucella abortus and Brucella ovis antigens, the complement fixing antibody titres were, however, higher with ...

Mice infected with Brucella abortus 19 were challenged intravenously with Listeria monocytogenes. Spleen assays to determine the number of viable Listeria cells present revealed that these mice were highly resistant to Listeria when challenged on day 17 of the Brucella infection. Resistance was absent in mice challenged on the 5th day and was declining in mice challenged on the 33rd day. Resist...

BACKGROUND The Qinghai-Tibet Plateau (QTP) of China is an extensive pastoral and semi-pastoral area, and because of poverty and bad hygiene conditions, Brucella is highly prevalent in this region. In order to adequately prevent this disease in the QTP region it is important to determine the identity of Brucella species that caused the infection. METHODS A total of 65 Brucella isolates were ob...

background: brucellosis is one of the most common zoonosis in middle east and iran. objectives: the purpose of this study was genomic detection of brucella spp. in sero-positive dairy cattle. methods: we have collected 28,519 blood samples from cows during 2012-2013. samples were screened by slide and tube agglutination and 2-mercaptoethanol tests. samples with anti-brucella antibodies titer ≥ ...

Brucella abortus biotypes 1 and 2 were recovered from various tissues of a naturally infected cow. This result indicates a need for biotyping multiple isolates of B. abortus from several affected animals in a herd before the biotype of the infecting strain can be definitively established.

Compared with virulent Brucella abortus 2308, the isogenic htrA mutant PHE1 shows decreased resistance to killing by cultured murine neutrophils and macrophages and significant attenuation during the early stages of infection in the BALB/c mouse model. These findings further define the contributions of the htrA gene product to the pathogenesis of B. abortus infections.

Brucella abortus is a Gram-negative, facultative intracellular pathogen for several mammals, including humans. Live attenuated B. abortus strain RB51 is currently the official vaccine used against bovine brucellosis in the United States and several other countries. Overexpression of protective B. abortus antigen Cu/Zn superoxide dismutase (SOD) in a recombinant strain of RB51 (strain RB51SOD) s...

One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic species, B. melitensis and B. abortus. GI-3 encodes mostly unknown proteins. O...